273 
Seedlings of Zea Mais and Phoenix dactylifera . 
relied upon when used alone. It gives no indication of the acidity or 
alkalinity of the cell-contents, nor does it differentiate the granules in the 
cytoplasm from those in the nucleus. 
Z winter m ami s Fuchsin- Iodine- Green. Repeated attempts with this 
otherwise valuable stain were not successful in producing a single good 
preparation. The stain seems to have no affinity for the granules, and not 
even the protoplasmic structures were stained satisfactorily. 
A nilin- Gentian- Violet-1 odine-Eosin . Grants Method. The sections 
were not stained deeply enough when this stain was used to afford any 
satisfactory study. 
Mantis E osin- T oluidin Blue. This proved to be the most valuable 
stain used, and was employed more extensively than any other in my work. 
The method described by Huie (’97) was employed in staining the sections. 
It not only differentiates cell-walls and cell-contents, but differentiates one 
kind of granules from another in the same cell. The cell-walls were stained 
blue ; starch-grains were stained bluish-green ; cytoplasm, blue (red in cells 
where secretion had progressed for some time) ; zymogen granules, blue 
(or purple) ; chromatin, reddish-purple. The best results were obtained in 
material which had been fixed by Mann’s aqueous picro-corrosive killing 
fluid. 
Eosin and Anilin Blue. This stain was used to good advantage with 
sections of Zea> where a differentiation of starch- and proteid-grains was 
desired. 
Eosin and Gentian Violet. The same methods were employed with 
this stain as for the two preceding, but it did not give as good results. The 
cell-contents were not plainly differentiated. 
Flemming s Triple Stain. Good preparations were obtained by the use 
of this stain, but it did not differentiate the different granules in the cell 
sufficiently to make it a valuable stain. It works best after the use of 
Chromo-osmo-acetic acid, but does not give good results after the use 
of fluids containing mercuric bichloride. 
III. Observations on the Scutellum of Zea Mais. 
A cross-section of the scutellum, when examined under the microscope, 
is seen to consist of large, nearly isodiametric cells, which are bounded on 
the side next the endosperm by a single layer of columnar epidermal cells. 
There is not only a noticeable difference in the size and shape of the two 
kinds of cells, but also in their contents. The granules found in the epi- 
dermal cells are always small, and are composed of proteid ; the granules 
in the large cells of the scutellum may be either starch or proteid ; and the 
proteid may be in the form of large or small granules. 
