276 Reed . — Z? Study of the Enzyme- secreting Cells in the 
Condition of the scutellum after thirty hours of activity (Fig. 3). The 
cells of the epidermal layer are noticeably larger than those in the stage 
last studied, and are completely filled with granular protoplasm, which 
is slightly denser at the distal end of the cell and stains purple with 
Mann’s Eosin-Toluidin Blue. The granules show some conformity in their 
arrangement to the strands of the cytoplasmic network. The nuclei are 
of the sort one finds in cells where active metabolism is going on. Nearly 
every nucleus is surrounded by a vacuole and lies in the proximal end 
of the cell. The karyolymph, or nuclear plasm, contains, as in the last 
stage, two kinds of material. However, there is much less of the fine 
granular material than in any preceding stage, and the larger bodies of 
nucleo-proteid, chromatin, have increased in size. It can also be seen that 
the chromatin is arranged on the linin network. The nucleolus, although 
distinct, is not prominent. The staining properties of the granules in the 
cytoplasm indicate that they are of a different character from those in the 
nucleus ; the former stain blue with Mann’s Eosin-Toluidin Blue, the 
latter stain red. Part of the proteid granules have disappeared from the 
more deeply lying cells of the scutellum. There is a slight increase in 
the amount of starch in the scutellum, the greatest increase being near 
the plumule. 
Morphology of the cells at the end of two days of activity. Radicles 
1 cm. long. There is a further decrease in the amount of the granular 
substance in the cytoplasm and an increase in the amount of chromatin 
in the nucleus (Fig. 4). The nucleolus is hardly to be distinguished because 
it has become smaller and lost most of its ability to absorb stain. The 
nucleus is usually located in the proximal half of the cell. The con- 
tents of the subepidermal cells of the scutellum are beginning to diminish. 
They are probably being used for the nourishment of the plant or to 
form enzymes. 
Morphology of the cells at the end of the third day of activity. Tem- 
perature 35 0 . On the third day the scutellum appears to be secreting 
diastase very actively. The epidermal cells contain a fine granular 
substance which stains bluish-purple and contains embedded in it larger 
granules, which are of almost the same size as the chromatin granules 
of the nucleus, but differ from them in staining properties. This granular 
matter is quite evenly distributed throughout the cell, but is nowhere 
so dense as in the preceding stages. 
The nuclei are at the middle of the cell or in the proximal half. The 
finely divided granular substance which was present in the nucleus has 
all disappeared at this stage, but the chromatin of the nucleus continues 
to increase. It exists in the shape of spherical and rod-like masses at 
the surface of the nucleus (Fig. 5). I have not seen any cases where 
I thought that solid particles were passing from the nucleus into the 
