6i6 Wolfe . — Cytological Studies on N emotion . 
Each cell of the antheridial branchlet usually gives rise to four antheridia 
which bud out radially, although even here the number is not constant. 
Fig. 37 represents a median section of such a branch in which only those 
antheridia developed laterally are shown. 
The divisions of the nucleus of the mother-cell from which these 
antheridia arise correspond to those of other vegetative cells in all respects, 
and the chromosomes, as usual, are about eight in number. The minute- 
ness of the figure necessarily renders accurate counting a difficult matter, 
but so many were seen in both longitudinal and polar view, and the 
cytoplasm is so clear at this time, that there is little reason to doubt 
that this estimate is approximately correct. The spindle is in no way 
peculiar, except that it is smaller and details are relatively even more 
difficult to differentiate than in the case of other cells of the plant. The 
spindle appears to be intranuclear, as is certainly the case in other mitoses. 
A centrosome was occasionally distinct at the poles of the spindle at 
metaphase (Fig. 39), but could not be made out in the majority of the 
cases examined (Fig. 38). 
The chromatophore divides after the nucleus (Fig. 42), and for a short 
time can be recognized as a faintly staining body, with a not very definite 
outline in the antheridium (Fig. 43). Soon, however, the contents of the 
cell undergo a remarkable reorganization, after which it is no longer 
possible to recognize any differentiated bodies in the protoplasm, except 
the nucleus itself (Fig. 47). In this reorganization the chromatophore 
disappears and a mass of densely staining matter is seen at the distal 
end of the antheridium (Fig. 44), a portion of which seems certainly to 
be derived from the disorganization of the chromatophore. For a time 
the nucleus is seen lying below this mass, but very soon is indistinctly 
visible in its midst (Fig. 45). This matter begins to stain less deeply, 
and well-marked, deeply staining, fairly uniform granules, twenty to 
thirty in number, appear on the nuclear wall. These granules suggest 
those bodies figured as chromatophore-derivatives by earlier writers (Figs. 
44-46). Although the phenomenon bears a striking resemblance to the con- 
ditions seen in the early prophase of ordinary mitoses (see Figs. 54 and 55), 
it is evident that it cannot be thus interpreted as a prophase character, 
from the fact that the nucleus resumes again its normal organization 
preparatory to the succeeding mitoses (Fig. 16, the spermatium at the left). 
As to the origin of these granules, it seems more reasonable to suppose 
that they represent food material, a part of which at least is derived 
from the chromatophore, and is now passing into the nucleolus. It should 
here be stated as further evidence in support of this interpretation that 
preparations of the cell at this stage (Fig. 47 in median section, and 
Fig. 48 in surface view), as well as in the stages immediately preceding, 
show that the nucleolus retains its normal size and appearance (Figs. 
