627 
Wolfe. — Cy to logical Studies on N email on. 
(5) In earlier stages the trichogyne possesses a well-organized nucleus, 
which fragments as that organ matures ; and, the egg-cell thus becoming 
an intercalary cell, the trichogyne must be regarded as a cell which has 
been specialized in connexion with the reproductive processes, and further, 
(6) The contents of the spermatium normally divide into two 
fertilizing elements which are discharged into the trichogyne : these events 
constitute this so-called spermatium an antheridium. 
(7) Fertilization consists essentially in the union of a male and 
a female gamete, and from the further divisions of the resultant fusion- 
nucleus the cystocarp arises after the separation of the stalk-cell. 
(8) In the mature cystocarp the ultimate cells give rise terminally, 
as well as by subterminal proliferation, to a variable number of carpo- 
spores, which is further augmented by repeated proliferation within the 
successively formed mother-cell walls. 
(9) In the nucleus the entire chromatin content is stored in the 
nucleolus, and in the prophases of division passes to the nuclear wall along 
delicate fibrillae. 
(10) The spindle is intra-nuclear, and centrosomes are distinctly visible 
at the poles at metaphase. 
(11) The conclusion that Nemalion presents the essentials of an 
antithetic alternation of generations, and that the cystocarp is, therefore, 
the homologue of the sporophyte in higher plants, is indicated by the 
cytological evidence — since 
(12) Approximately sixteen chromosomes are present in the divisions 
of the cells of the cystocarp up to the period of spore-formation, and 
approximately eight in those of the thallus ; the reduction division being 
immediately associated with the production of the carpospores. 
Literature cited. 
Bornet and Thuret (’67) : Recherches sur la fecondation des Floridees. Ann. Sci. Nat. Bot, 
5 e ser., tome vii, pp. 137-166, PI. 3. 
— (’76) Notes algologiques. Paris. 
(’78) : Etudes phycologiques. Paris. 
(’80): Notes algologiques. Paris. 
Boubier, A. M. (’99) : La membrane pyrenoidienne. Bull, de l’Herbier Boissier, vol. vii, 
pp. 451-458, Text Figs. 
Chmilowsky, W. (’97) : Ueber Bau und Vermehrung der Pyrenoide bei einigen Algen. (Rev.) 
Bot. Cent., vol. lix, pp. 277-278 (1897). 
Davis, B. M. (’ 96) : The Fertilization of Batrachospermum. Ann. Bot., vol. x, pp. 49-76, Pis. 6 and 7. 
(’99) : The Spore-mother-cell of Anihoceros. Bot. Gaz., vol. xxviii, pp. 89-108, 
Pis. 9 and 10. 
