Wolfe . — Cytological Studies on N emotion . 629 
Fig. 4. The egg-nucleus is now above the chromatophore — an unusual position. The primary 
egg-nucleus has probably divided, giving rise to the egg-nucleus, and to that of the trichogyne. The 
wall of the trichogyne is now, and also in later stages, very thin at the tip. 
Fig. 5. The egg-nucleus has resumed its normal position below the chromatophore and in the 
basal portion of the cell. 
Fig. 6. The trichogyne is elongating, and the nucleus is still intact. 
Fig. 7. Fragmentation of the nucleus of the trichogyne is beginning to take place. 
Figs. 8-10. Later stages in the process of fragmentation. The fragments gradually stain less 
deeply. A thin wall surrounds the entire receptive portion of the trichogyne. 
Fig. 11. The carpogonium is now ready for fertilization, and shows what are probably the last 
traces of a disorganizing nucleus. 
Fertilization and Development of the Cystocarp. 
Fig. 12. An early stage in fertilization. Residual protoplasm and fragments, probably, of the 
second male nucleus, are seen in the spermatium, which has conjugated with the trichogyne. The 
male nucleus is in the top of the carpogonium, while the egg-nucleus is still in the basal portion. 
The protoplasm of the trichogyne has shrunk away from the thin wall, and is beginning to 
disorganize. 
Fig. 13. Actual fusion of the male and female nuclei, the male the smaller of the two. Its 
nucleolus is at one side. The nuclear walls are more distinct than usual. 
Fig. 14. A similar stage. Apparently a second nuclear body lying beside the chromatophore. 
Fig. 15. The same stage. 
Fig. 16. The fusion-nucleus now lies in the top of the carpogonium. The separation of the 
trichogyne is beginning to take place. Three spermatia are in contact. The one at the left has not 
fused, and its nucleus has not divided. The empty one has probably given rise to two sperms, one 
of which has fused with the egg-nucleus, and the other may still be seen midway the trichogyne. 
The third spermatium has just discharged one of its sperms, and the other is still within the 
mother-cell. 
Fig. 17. A wall is now formed around the zygote, and the fusion-nucleus has resumed its usual 
position at the base. 
Fig. 18. Division of the zygote into a stalk and a sporogenous cell. The chromatophore has 
already divided. 
Fig. 19. Somewhat later. 
Fig. 20. Similar to 17. Granular masses appear in the hypogynous cell. 
Fig. 21. First division of the sporogenous cell. The nucleus of the cell to the right is in a lower 
focus. The dark mass is probably the chromatophore. The chromatophores of the stalk and 
hypogynous cells are disintegrating. 
Fig. 22. A section of a young cystocarp at about the six-celled stage. The chromatophores are 
now distinctly differentiated. 
Fig. 23. Gonimoblasts. The terminal cells are separating as spores. Chromatophores are 
present in all the cells. 
Structure of the Chromatophore. 
Fig. 24. The chromatophore may be seen to consist of a central ellipsoid, from which processes 
radiate to the periphery of the cell, and there flatten out into a clathrate membrane. 
Fig. 25. An absolute median section through the chromatophore, showing the interior of the 
ellipsoidal body to be devoid of protoplasmic contents. 
Division of the Chromatophore . 
Figs. 26 and 27. Processes extending into the new cell. 
Fig. 28. Illustrating a rapid division of the chromatophore independent of the nucleus. 
Fig. 29. The central body is bulging out into the daughter-cell. 
Fig. 30. The bulge has increased until the two together present the appearance of a single 
elongated chromatophore. 
Fig. 31. Constriction beginning. 
