On the Fruits and Seeds of Rhainnus. i 9 
No. 11. To a sixth portion of the pericarp solution added 
nothing. 
It remained unaltered and clear for two days. 
It had now become clear that (1) the ferment is contained 
somewhere in the seed ; (2) it is confined broadly to the testa 
or outer coat of the seed ; and (3) moreover is localised — 
situated somewhere in or near the thickened margins where 
the testa is turned in. (4) The ferment is not destroyed by 
keeping for two years. 
I now repeated many of the experiments given above, with 
the seeds and fruits of the same species ( Rhamnus infectorius ) 
but from another source, and which had not been kept so 
long. I first dissected twenty-four seeds, under the simple 
microscope, separating the thickened margins of the testa 
(where it is turned in) from the rest of the seeds — i. e. the 
testa of the sides, the endosperm, and the embryos. 
Series V. 
y. An aqueous extract of the thickened inturned margins of 
twelve seeds. 
Yielded a perfectly clear colourless liquid. 
6. Aqueous solution of the rest of the twelve, seeds (i. e. the 
endosperm, embryos, and the testa from the sides of the 
seeds). 
Yielded a clear, colourless liquid h 
r\. A glycerine extract of the thick inturned margins only 
of twelve seeds. 
Perfectly clear extract. 
6 . A glycerine extract of the rest of the seeds. 
Clear solution. 
I then added a few drops of each of these extracts to a 
series of test-tubes containing fresh boiled solutions of the 
glucoside (xanthorhamnin) obtained from the pericarps. 
1 Faint tinge of yellow. 
C 2 
