230 Green.— On the germination of the tuber of 
collected and concentrated over water baths till of very small 
bulk, when they had a syrupy appearance and consistency. 
These concentrated dialysates were found to contain three 
bodies that could be separated from each other by treatment 
with alcohol. The first of these was a sugar, and was 
separated by extracting the syrupy residue with absolute 
alcohol, when about half of it dissolved. On decantation from 
the undissolved residue, and concentration over a water bath, 
this again became syrupy, and remained so, refusing to 
crystallize, even when exposed over strong sulphuric acid. It 
was freely soluble in cold water, and its solution reduced 
Fehling’s. fluid when boiled with it. As I was unable to get 
it into crystalline form, I could not determine its specific 
rotatory power. It had a feebler reducing power than dextrose 
or laevulose, and this power was considerably increased by 
boiling it for a few minutes with about two per cent, of HC1. 
Having extracted this sugar from the concentrated dialy- 
sates by treatment with absolute alcohol, there remained a 
residue about equal in bulk to the sugar taken up. A great 
deal of this dissolved freely in cold water, which is not the 
case with unaltered inulin. The rest remained insoluble till 
heat was applied. This consisted of inulin that had dialysed 
through the parchment during the later stages of the digestion. 
An experiment was conducted on this power of dialysis, 
some inulin-solution, without any ferment, being dialysed in a 
fresh well-tested parchment tube for several days, when the 
dialysate, on being concentrated, deposited a residue which 
the microscope showed to consist chiefly of the well-known 
sphaero-crystals of inulin. 
The two constituents of the residue, after separation of 
the sugar, were separated from one another by treatment 
with alcohol. Careful experiments showed that inulin was 
insoluble in alcohol of sixty-five per cent, strength. On 
making the solution of the residue up to this strength of 
spirit, there was a precipitate which gradually separated out 
and settled to the bottom of the fluid. On filtering and adding 
further alcohol, no change took place till about eighty-two per 
