346 Ward. — Recent Ptiblications bearing on the 
the early phases were passed through, and in the second 
week of June the aspect of the plants was changing, and 
they became pale as the reserve-materials were exhausted. 
So far, there was no difference to be observed between the 
forty cultures. 
On June 13, however, a difference began to set in, and 
by June 18 it was decided — ‘In the ten vessels supplied 
with bacteria 1 , all the plants had regained their fresh green 
colour, and commenced to grow vigorously.’ 
Of the thirty vessels in which the appearance of micro- 
organisms was left to chance, only two at this time presented a 
similar appearance, the remainder starving and in part yellow. 
By the 30th of June, the plants supplied with bacteria 
were developing the tenth leaf, and were luxuriant ; only 
one of the twenty individuals was behindhand, and the deep 
green colour showed this was not from want of nitrogen- 
later examination showed that its tap-root was injured. 
Of the sixty plants not supplied with bacteria, about ten 
were nearly as flourishing as the above, and five were nearly 
dead : among the remaining forty-five were all stages between 
these extremes. 
At this time the plants from two of the vessels infected 
with bacteria and those from five of those not so infected were 
taken up and examined, and showed the above-described 
relation between the growth of the sub-aerial parts and the 
development of the root-tubercles. 
Of twenty-two plants to which no bacteria were added, 
only five yielded more than 15 gr. dry substance, as follows — 
No. 2=15*053^ 
„ 26=15.950! 
„ 29 = 17.142' gr. 
„ 18 = 17.3051 
„ 1 = 20-372] 
The yield of the remaining seventeen plants was between 
1*640 gr. to 13*190 gr. 
1 Here again it is of course an assumption that ‘ bacteria ’ were the agents. 
