Buller . — The Enzymes of Polyporus squamosus , Huds . 53 
The results in the above table indicate that the Fungus extract is 
capable of breaking down Witte peptone so that the products give the 
reaction for tryptophane. We may therefore conclude that the Fungus 
extract contains a protease which, according to Vines, may be considered 
to be an erepsin. 
Bourquelot and Herissey (loc. cit.) found a feebly proteolytic enzyme in 
Polyporus sulphur eus . In respect to this enzyme, then, Polyporus squamosus 
behaves similarly. 
(5) Emulsin. 
TABLE III. 
No. 
Extract. 
Amygdalin 
5 per cent. 
Antiseptic. 
Prussian blue reaction after 
Smell after 
40 hours. 
2 3 hours. 
40 hours. 
Experiment 
1 
1 c.c. 
5 c.c. 
toluol 
distinct 
green 
distinct green fol- 
lowed by a blue 
precipitate. 
strong of HCN and 
benzoic aldehyd. 
Experiment 
2 
I c.c. 
5 c.c. 
benzene 
very faint 
green 
faint green followed 
by light blue pre- 
cipitate. 
distinctly of HCN 
and benzoic alde- 
hyd. 
Control . . 
3 
I c.c. 
boiled. 
5 c.c. 
toluol 
yellow 
yellow : no trace of 
blue. 
none. 
The particulars of the experiments, made in test-tubes at about 18 °C 
are embodied in the above table. The Prussian blue and smell tests clearly 
indicate that the fruit-bodies contain an emulsin enzyme. 
(6) Rennetase. To ioc.c. of fresh milk 2 c.c. of Fungus extract were 
added in a test-tube a. The control was made in a similar manner, the 
extract, however, being first boiled. The tubes were placed at a temperature 
of 28° C. 
After forty-five minutes the contents in a had coagulated, whereas in 
the control there was no coagulation at all. 
A few grams of a fruit-body, which had been carefully dried in thin 
slices, were pounded up with water and sand. The extract was forced 
through a cloth. Similar experiments to those just described were then 
made. 
Clotting of the milk was seen to have begun fifteen minutes after the 
beginning of the experiment. At the end of two hours there was a solid 
plug at the top of the tube. The control remained unclotted. 
The above experiments clearly prove that the fruit-bodies contain 
a rennetase. 
(7) Lipase . The extract from 100 gm. of the Fungus pounded up with 
icoc.c. water was 164 c.c. The tests were made upon a solution of 1-84 per 
cent, ethyl acetate. The 50 c.c. flasks employed were corked and placed in 
