54 B uller . — The Enzymes of Polyp or us squamosus , Huds. 
a warm chamber at 28° C. Litmus solution was used for determining 
neutrality. The other details of the experiments are given in the table. 
TABLE IV. 
Experiment 
Acetate 
solution. 
Extract 
Toluol. 
Amount of ^ normal KHO solution in c.c. 
required for neutralizing io c.c. after 
40 hours. 
51 hours. 
93 hours. 
330 hours. 
40 c.c. 
10 c.c. 
0-5 c.c. 
3-8 
1 1.06 
15-5 
18.4 
Control 
40 c.c. 
10 c.c., 
boiled 
0.5 c.c. 
0.14 
0.12 
0.15 
o*3 
Excess KHO in experiment over control by 
subtraction 
after 
40 hours. 
after 
51 hours. 
after 
93 hours. 
after 
330 hours. 
3/6 
10.94 
1 5 • 3 5 
18.1 
Grams of ethyl acetate hydrolyzed in ioc.c. 
by calculation 
0.0161 
0 
6 
4- 
00 
0.0631 
0.0796 
It is evident from the results given in the above table that the ethyl 
acetate underwent continual hydrolyzation, 43 percent, thus becoming split 
up in 330 hours. In the control there was practically no hydrolyzation. 
The presence of a lipase in the Fungus extract is thus made highly probable. 
It must not be forgotten, however, that a lipase is an enzyme, which splits 
up fats, and not necessarily such esters as ethyl acetate. It is as yet not 
absolutely certain that such an alcohol as ethylic can replace glycerine 
in the molecule so as to be acted upon in the same manner by lipase. 
Provisionally, however, it seems to me best to retain the name lipase 
for those enzymes which have the power of splitting up such compounds as 
ethyl acetate. 
It may here be mentioned that when spores of Polyporns squamosus 
are allowed to dry for several days, many of them develop large fat-drops. 
On germination of the spores in malt-wort extract these drops disappear. 
Perhaps this is due to the action of a lipase. 
(8) Pectase. The juice was squeezed out of fresh, ripe, red currants, 
filtered, then boiled and refiltered. Excess of alcohol was added to pre- 
cipitate the pectin. This was then filtered as dry as possible with the aid 
of a suction filter. Water was then added to dissolve the pectin and the 
solution placed on a warm bath to drive ofif the alcohol. This solution was 
then used in testing for pectase. 
The following were preliminary experiments to assure myself that the 
solution contained pectin. To 1 c.c. freshly extracted carrot-juice, which is 
known to contain pectase, 5 c.c. of the pectin solution were added in a 
