56 Biiller . — The Enzymes of Polyporus squamosus , Huds. 
porus sulphur eus , for Bourquelot and Herissey (loc. cit.) were unable to 
detect the enzyme in that species. 
(10) Maltase. One gram of dried slices of the Fungus were well rubbed 
up with sand and a little of a 2 per cent maltose solution. Of this mixture 
half by weight was put into each of two 100 c.c. flasks which were then filled 
up to the 100 c.c. mark with 2 per cent, maltose. The contents of one flask 
were boiled in order to make a control experiment. One c.c. toluol was 
used as an antiseptic in each case. The flasks were placed in a warm 
chamber at 29 0 C. 
At the end of three days 25 c.c. were removed from each flask, mixed 
with aluminium hydrate, and then filtered. The clear solutions thus 
obtained were then tested in the polarimeter. The readings were almost 
identical. 
The experiments indicate that the fruit-bodies do not contain maltase. 
(11) Trehalase . Some trehala manna was obtained for me by Pro- 
fessor Adrian Brown through the kindness of the Pharmaceutical Society. 
From the manna a small amount of trehalose was prepared. The Fungus 
extract was made by rubbing up 5 grams of the dried fruit-bodies with 
50 c.c. of water. 
To 10 c.c. of a 3 per cent, trehalose solution 1 c.c. of extract was added. 
A polariscope reading was then taken. Further polariscope readings were 
taken at intervals for three days, but no change was observed. The 
experiment, therefore, seems to indicate that no trehalase is present in the 
Fungus extract. However, Bourquelot and Herissey (loc. cit.) found that 
this enzyme is present in the fruit-bodies of Polyporus sulphureus. It 
is, therefore, somewhat surprising that Polyporus squamosus does not seem 
to contain it. 
(12) Cytase. As a preliminary experiment and control 20 gm. of 
oats, which are known to contain cytase, were ground up, mixed with 
water, and after two hours filtered. The extract was then allowed to 
act on thin sections of barley-grains which had been cleared of starch 
in the course of twenty-four hours by means of saliva. The sections 
were watched in hanging drops under the microscope l . After two 
hours the cellulose walls of the sections were seen to be swelling and 
dissolving away, proving that a cytase had been extracted from the oats. 
Similar experiments were made with a Polyporus extract, but with 
negative results, so that I was unable to prove the presence of a cytase 
in the fruit-bodies. Owing to the disappearance of cellulose from the 
walls of the cells in the wood of the Sycamore under the action of the 
Fungus 2 there can, however, be little doubt that the vegetative part at 
least produces abundant cytase. 
(13) ‘ Coagidase .’ According to the recent work of Wolff and 
1 See Marshall Ward, A Lily Disease, Ann. of Bot., vol. ii, 1888. 2 Buller, loc. cit. 
