7 2 
Pond . — The Incapacity of the 
in water. It does not respond to any of the tests for proteids. This 
behaviour excludes albumins and globulins, and adds emphasis to the 
negative tests with the extract itself. These observations do not agree 
with those of Puriewitsch (’97, p. 66) who obtained flocculation on boiling 
his ‘ culture fluid ’ with acetic acid, and a white precipitate with acetic acid 
and potassium ferrocyanide. Either his observations were faulty or else 
some coagulable proteid passed from the embryo to the endosperm during 
germination. 
Extraction with sodium chloride . The same powder, after the preceding 
extraction with water, was digested with 5 per cent, sodium chloride for 
sixty hours (toluol, 5 per cent.). This extract was neutral to litmus. On 
heating, a milky turbidity appeared which was more opaque than was that 
of the aqueous extract. The heating was continued to boiling for a 
minute, when a flocculent precipitate appeared which redissolved on cooling 
and reappeared on heating. The turbidity was destroyed by acetic acid 
and did not reappear until the acidity was neutralized by ammonium 
hydroxide. Other reactions agreed with those of the aqueous extract, 
except that a white precipitate formed with acetic acid and potassium 
ferrocyanide. This latter result was found to be due to impurities in the 
toluol, as the acetic acid alone with the toluol gave the white precipitate. 
The flocculation on boiling was found to be due to impurities in the sodium 
chloride. This leaves the sodium chloride extract negative in all tests for 
proteids, just as was the aqueous extract, and positive for phosphates. The 
sodium chloride itself did not react for phosphates with molybdic solution. 
Extraction with sodium hydroxide , -i per cent. The residue remaining 
after the extraction with sodium chloride was digested sixty hours with 
sodium hydroxide, -i per cent, and toluol, 5 per cent. The filtered extract 
was found to be only slightly alkaline so that the alkalinity had evidently 
decreased during the digestion. This extract does not have the opalescent 
hue of the other extracts but instead is of a deep amber colour. This colour 
is undoubtedly due to the alkali, as it appeared almost instantly when the 
latter was added. As the neutral point was slowly approached with acetic 
acid the amber gradually disappeared, being replaced slowly by a dirty 
opalescence. As the reaction became faintly acid a cloud was noticed, 
which gradually spread through the liquid as the reaction became distinctly 
acid. On standing a few minutes this turbidity developed to a flocculation 
and a curdy precipitate finally settled. 
This precipitate changed to brick red on boiling with Millon’s reagent. 
The filtrate did not react with any reagents for proteids nor with ammo- 
nium sulphate. The extract itself did not respond to any of the proteid tests 
except with ammonium sulphate. The latter test was positive because the 
small amount of proteid present was concentrated into a precipitate. 
Coagulation did not occur when the extract was boiled at the neutral point. 
