Ellis . — The Life-history of Bacillus hirtus. 
257 
Literature. 
1. Ellis : On the discovery of cilia in the genus Bacterium. Centralblatt fur Bakteriologie, 
Bd. xi, 1903, No. 8-9. 
2. — — : Der Nachweis der Geisseln bei alien Coccaceen. Centralblatt fiir Bakteriologie, 
Abt. II, Bd. ix. 
3. : Untersuchungen fiber Sarcina, Streptococcus und Spirillum. Bd. xxxiii, 1902-3. 
4. Fischer, A. : Vorlesungen iiber Bakterien. Jena, 1897. 
5. ; Untersuchungen iiber Bakterien. Pringsheim’s Jahrbiicher fiir wissenschaftliche 
Botanik, Bd. xxvii, Heft 1, 1895. 
6. Gottheil, O. : Botanische Beschreibung einiger Bodenbakterien. Centralblatt fiir Bakteriologie, 
Bd. xxxi, 1902. 
7. Henrici : Beitrag zur Bakterienflora des Kases. Arbeiten aus dem bakt. Institut der Techn. 
Hochschule zu Karlsruhe, Bd. i, 1894, Heft 1, p. 44. 
8. Jackson, D. D. : Paper read before New York Microscopical Society, April, 1905. 
9. Meyer, A. : Studien iiber die Morphologie und Entwickelungsgeschichte der Bacterien aus- 
gefiihrt an Astasia asterospora, A. M., und Bacillus tumescens , Zopf. Flora, 1897. 
10. : Ueber Geisseln, Reservestoffe, Kern- und Sporenbildung der Bakterien. Flora, 1899. 
11. Migula, W. : System der Bakterien, vol. i, Jena, 1897. 
EXPLANATION OF PLATE. 
Figs. 1-4, 6, 10, 15-20, 23-29, 48-49 drawn with drawing apparatus. Magnification 1650. 
Fig. 1. Spore stained with Iodine, showing intine strongly and exine faintly coloured. 
Figs. 2-3. Spores stained with Methylene-blue. 
Fig. 4. Spore stained with Fuchsin, exine coloured, intine untouched by stain. 
Fig. 5. Spore very highly magnified to show exine and intine. 
Fig. 6. Spore stained with Methylene-blue seen ‘ end on.’ 
Figs. 7-9. Stages in germination of the spore. 
Fig. 10. Appearance of individuals in a five hours old culture. Chains of two-celled 
individuals. 
Fig. 11. A two-celled individual. Appearance of individuals when growing under optimum 
conditions of growth. 
Fig. 12. Portion of a filament in which very prolific formation of spores has taken place. 
Figs. 13-14. Gelatine stab-cultures. See text for explanation. 
Fig. 15. Individuals from Nutrient medium No. 1 culture. 
Figs. 16-17. Individuals from Agar culture. Fifteen hours old. 
Fig. 18. Cilia preparation from a two days old Agar culture. 
Fig. 19. Spore containing individual stained with Fuchsin, in which abundant cytoplasm is 
found in the vegetative cell, even after the spore is completely ripe. 
Fig. 20. Cilia-preparation from a four days old Agar culture. 
Fig. 21. Schematic representation of length of individuals from a twenty-four hours old Agar 
culture. The transverse line indicates cell-division at that point. 
Fig. 22. Same from a two days old culture. 
Fig. 23. Two-celled individual in which a division wall is formed, before elongation has 
taken place. 
Fig. 24. Two-celled individual in which a division wall is formed after very slight elongation. 
Figs. 25-34. Stages in cell-division. For explanation see text. 
Figs. 35-44. Stages in spore development. For explanation see text. 
Figs. 45-46. Micro-photographs taken with 1/12 oil-immersion lens to show distribution of 
cilia. Cilia stained with Night-blue. Kindly photographed by Mr. Garry. 
Fig. 47. Cell stained with Fuchsin to show cellular membrane. 
Fig. 48. Plasmolysed cell which separates the cell-wall from the protoplasm. 
Fig. 49. Cell stained with Fuchsin to show cellular membrane. 
