390 
A T otes. 
twenty-four hours. Professor Vines informs me that similarly in- 
soluble proteids occur in the form of aleurone-grains in the seeds of 
Musa Hillii. But I have no doubt that Liidtke and Schenk, in their 
work, mistook the globoids for the intact aleurone-grains. Hence 
their statements of the insolubility of these grains of the aleurone-layer 
by peptic ferments and in dilute acids (Schenk), or in caustic potash 
(Liidtke). 
By means of identical methods I worked out the distribution of 
globoids in the embryo of the seeds investigated. 
Special Work. 
Coix Lachryma . — The aleurone-layer is but one cell thick. Each 
aleurone-grain consists of a shell of proteid matter enclosing a single 
central globoid. Oil occurs throughout the whole of the embryo, and 
in the aleurone-layer, but not elsewhere in the endosperm. Globoids 
are found abundantly in the embryo and in the aleurone-layer \ 
If the microchemical methods adopted be correct, we should 
expect a chemical analysis to show that the embryo is rich in 
phosphates, in magnesium, and calcium — corresponding to the vast 
number of globoids present in most of the cells composing the 
embryo. We should anticipate that there would be less ash in the 
endosperm — corresponding to the fact that it is mainly composed of 
starch, with but little proteid matter, and with globoids only occurring 
in the outermost layer. We should expect that this ash would contain 
phosphates, magnesium, and calcium, in considerable quantities : but 
that the calcium would be present in relatively larger quantities in 
connexion with the great amount of carbohydrate material, quite 
apart from the globoids. 
And analysis reveals the fact that our anticipations are correct. 
For this analysis I am indebted to Mr. J. J. Manley, of the Chemical 
Laboratory, Magdalen College, Oxford. It will be seen that no 
account is taken of the potassium present. 
Endosperm. Embryo. 
Water and organic matter . 99-4954% 88-71517, 
Ash . -5046 11-2849 
100 100 
1 In neither this seed nor in any other could I establish the presence of globoids 
in the epithelial layer of the embryo. But in Coix minute granules occur in the 
protoplasm of the epithelium ; and these appear to answer the tests for globoids as 
far as their minute size enabled me to judge. 
