494 Wager. — On Nuclear Division 
size, the original nucleolus in the protoplasm becomes smaller, 
until at last it completely disappears. The two daughter- 
nuclei divide again in the same way, as do the four nuclei 
produced by this second division, until the eight nuclei are 
produced. 
I will now give an account of my own observations. 
Methods. 
The following is a short account of the methods employed 
in this investigation. 
The fungi were cut up into short pieces, as soon as possible 
after they were collected, which were placed in a saturated 
solution of corrosive sublimate in which they were left for 
about twelve hours ; they were then washed well in water and 
gradually brought into methylated spirit through 30, 50, and 
70 per cent, spirit. They were next passed successively 
through absolute alcohol, xylol, and paraffin, and embedded 
in the usual way in blocks. The pieces of tissue were not 
left in the paraffin longer than was sufficient to allow the 
complete penetration of the latter, which was kept at as 
low a temperature as possible. Moderately thin ribbons 
of sections were cut by the microtome. These were cut into 
short lengths, flattened by dropping them carefully on to the 
surface of warm water and were then taken up on glass slips 
and allowed to dry. By this means the sections became 
attached sufficiently firmly to the slip to render cement 
unnecessary. The slides were then warmed just enough to 
melt the paraffin, and placed at once in turpentine or xylol to 
remove the paraffin. They were then brought into absolute 
alcohol to get rid of the turpentine or xylol, and were finally 
placed, ready for staining, in methylated spirit. 
Numerous staining methods were employed and it was 
found that all the ordinary stains such as haematoxylin, 
carmine, safranin, & c., coloured the nuclei more or less deeply, 
but it was found that none of these stains brought out the 
structure of the nucleus distinctly ; moreover, it was impossible 
by means of them to get any clear indication of nuclear 
