5 1 8 Massee . — On Trichosphaeria Sacckari, Mass.; 
ing up to 8 n diameter and full of brilliant, fine grained, 
homogeneous protoplasm. These thick hyphae originated as 
lateral branches from the delicate hyphae first produced by 
the conidia. No trace of fusion of hyphae was observed in 
any of the numerous cultures examined, when grown in sugar- 
cane solution, whereas in every other nutrient solution used, 
conjugation of the hyphae, brought about by the curving of 
a branch as described by Ward 1 , were abundant. I have 
frequently observed in cultures of other fungi that conjugation 
of the hyphae is more abundant when grown in a nutrient 
solution differing materially from the one most closely allied 
to the normal condition of life of the species, i. e. a solution 
of the host or substratum. The addition of a one per cent, 
solution of cupric sulphate killed the mycelium in flask 
number 2. 
After twelve days’ growth the contents of the third flask 
assumed a dark olive colour and the entire surface of the 
mycelium at the level of the solution presented the appear- 
ance of an olive-coloured, dense, velvety pile. The velvety 
appearance proved to be due to the presence of closely packed, 
erect, dark olive conidiophores growing out into the air. each 
bearing at its apex a single chain of reddish-brown conidia — 
to be spoken of in future as microconidia. The dark colour 
of the mass of mycelium immersed in the fluid was found to 
be due to immense numbers of large conidia arranged in 
chains and springing from the tips of the thick hyphae pre- 
viously described. These last will be distinguished as macro- 
conidia,) and, along with the microconidia, will be described in 
detail later. 
Small portions of sugar-cane, containing hyphae of the 
Macrosporium, were placed in the nutrient solution, and in 
twelve days the liquid was crowded with mycelium bearing 
both forms of conidia mentioned above. As illustrating the 
vitality of the hyphae, it may be mentioned that thirteen 
weeks after the diseased canes were received at Kew, a small 
1 A lily- disease ; Ann. Bot, Vol. II, p. 329 (1888). 
