522 Mas see. — On Trichosphaeria Sac chari, Mass.; 
produced on a piece of healthy Kew cane that was placed 
in a flask containing mycelium produced by Melanconium- 
conidia. 
Macroconidia. 
As already stated, pure cultures have proved that the 
hyphae developed from M elanconinm-c onidia eventually give 
origin to stout lateral branches which in turn bear apical 
chains of macroconidia. These stout branches of hyphae 
vary from 5-8 /x in diameter and give off numerous lateral 
branches at short intervals. When growing in a nutrient 
solution, some of these lateral branches grow to a great 
length before they branch, and have numerous transverse 
septa ; finally, a considerable number of short lateral branches 
appear at intervals, these are also transversely septate, and 
usually more or less curled or contorted and lobed, and 
undergo no further modification. An examination of the 
hyphae of this phase of the fungus growing in the tissues 
of a cane shows that these septate hyphae are vegetative 
in function, the main branch following the middle lamella, 
and giving off short lateral branches which enter the cells 
and act as haustoria. 
The short, sparsely septate or aseptate branches are filled 
with dense, finely granular protoplasm which contains no 
vacuoles until the apex of the hypha becomes slightly clavate, 
when the protoplasm becomes frothy and vacuolated. The 
apex of the hypha continues to swell until it is about twice 
the diameter of the hypha, when it gives origin to a monili- 
form chain of macroconidia produced in basipetal succession 
as follows (Fig. 8). If transverse septa are originally present 
they are absorbed previously to the formation of conidia. 
When the tip of the hypha has become sufficiently enlarged, 
the apex of the cell-wall swells up and finally disappears, 
the contained protoplasm slowly protruding through the 
cavity thus formed (Fig. 11). When the protoplasm has 
extended for a distance of 10-12 /x beyond the open apex 
of the mother-cell, a transverse septum appears in the portion 
