528 Massee.—On Trichosphaeria Sac chari, Mass. ; 
a chain at the ruptured apex of the conidiophore in a manner 
precisely similar to the macroconidia already described ; they 
are elliptic-oblong with truncate ends, of a clear pale reddish 
brown colour at maturity, and measure on an average 
10-11 x 6 fx. The number of conidia in a chain rarely exceed 
ten, and the terminal one is shaped like the rest, and not 
spherical as in the macroconidia (Fig. 10). 
Expt . I. Microconidia placed in a cavity made in a cane 
produced macroconidia in the interior of the cavity and 
microconidia at its surface. 
Ascigerous Stage. 
Two mature perithecia were found on a much decayed 
portion of one of the canes received from Barbados ; they 
sprang from a point that had previously borne a crop of 
microconidia, and were surrounded by old collapsed conidio- 
phores, the conidia having disappeared. Although the 
evidence in favour of a genetic connexion between the 
perithecia found on the cane and the microconidia with 
which they were associated was strong, yet it could not be 
accepted as conclusive; and it was not until similar perithecia 
were accidentally discovered on the surface of the material 
contained in one of the flask cultures, that this supposition 
was proved to be correct. The flask in question was one 
of which the contents were not required during the in- 
vestigation, and contained a dense mass of hyphae produced 
from a macroconidium. The submerged portion of the 
hyphae was black from a copious development of macro- 
conidia, while the surface was covered with a dense pile of 
conidiophores bearing microconidia. One day this flask was 
accidentally broken, and out of curiosity a portion of the 
contents, taken from the surface bearing microconidia, was 
placed under the microscope, when something much re- 
sembling a very young perithecium was seen. Further 
search revealed the presence of two young perithecia, almost 
colourless and without fruit, but bearing the long, charac- 
teristic bristle-like septate hyphae, present on the mature 
