532 Massee . — On Trichosphaeria Sacchari , Mass. 
Fig. 5. Conidia of Melanconium , two of which are germinating. X650. 
Fig. 6. Portion of a cane split down the centre, showing, a, the large internal 
black mass of macroconidia ; and b, the external microconidia forming a minutely 
velvety, blackish patch. Nat. size. 
Fig 7. Portion of the tissue of a cane showing the formation of, a , a' internal 
macroconidia ; b, external microconidia, borne in chains c , at the tips of long 
dark-coloured hyphae or conidiophores, d. x 650. 
Fig. 8. Portion of hypha producing macroconidia. Drawn from a pure culture 
grown in a sterilized solution of cane-sugar and produced from a macroconidium. 
Specimens grown under such circumstances are much larger than the same form 
when produced normally in the tissues of the cane ; some of the chains contain 
sixty-seven conidia, and the apical globose conidium is very large and distinct. 
X650. 
Fig. 9. Macroconidia germinating ; a , intercalary conidia ; b , terminal globose 
conidium. The conidia were obtained from a pure culture, x 650. 
Fig. 10. Microconidia germinating. The conidia were obtained from a pure 
culture, x 650. 
Fig. 11. Tips of hyphae giving origin to macroconidia ; a , showing the cell- wall 
at the apex of the hypha just commencing to deliquesce for the purpose of allowing 
the protrusion of the protoplasm which forms the terminal conidium of the chain ; 
b, the same further advanced, the protoplasm having grown out of the ruptured 
sheath and formed a cell-wall of its own. x 1000. 
Figs. 12, 13. Mycelium belonging to the macroconidium-stage present in cells of 
the fundamental tissue of the cane. In passing through the cell-walls, a , a', the 
pits in the wall are always used, a narrower portion of the hypha passing through 
and at once assuming the normal diameter on the opposite side, x 650. 
Fig. 14. A branch of mycelium that has sent two branches into a living cell; 
the branches have developed into lobed haustoria. X650. 
Fig. 15. A short lateral branch of mycelium showing at its tip the fundamental 
coil, a , preceding the formation of a perithecium. X650. 
Fig. 16. A very young perithecium still attached to the parent hypha, a. x 650. 
Fig. 17. A mature perithecium. X350. 
Fig. 18. One of the large coloured hairs from the external wall of the peri- 
thecium. x 500. 
Fig. 19. Asci from the perithecium in various stages of development ; one is 
mature and containing eight spores, x 650. 
Fig. 20. Ascospores liberated from the ascus (Fig. 19). Two of the spores are 
germinating, x 750. 
Fig. 21. Portion of a pellet of excrementa belonging to the cane-boring beetle, 
consisting of broken up mycelium and macroconidia, the latter are germinating. 
X650. 
