30 Barclay . — On the Life-history of 
life-cycle of the parasite from one year to another. But 
there can be no reasonable doubt that the teleutospores 
formed at the end of summer lie dormant until the following 
spring. 
These promising results induced me to continue my ob- 
servations in the following year. (Expt. 7.) — On the 2nd 
April, 1890, I collected some fresh spores and put them into 
water : on the following day there was no sign of germina- 
tion : from the 4th to the 7th I was absent from my labora- 
tory ; but on the 8th (24 X 6 hours), on my return, I found 
that they had germinated very freely, the water containing 
numerous sporidia. (Expt. 8.) — Again, on the nth I collected 
fresh spores and put them into water on the 12th. On the 
13th and 14th there was no germination : on the 15th (24 x 3 
hours) some had germinated, and there were some sporidia in 
the water; but it was not until the 19th (24x7 hours) that 
germination had become free, and the water held immense 
numbers of sporidia. (Expt. 9.) — Lastly, on the 19th April 
I collected more fresh spores and put them into water: on 
the 20th there was no germination : on the 21st a good many 
had germinated : on the 22nd (24 x 3 hours) germination was 
general. 
At the same time I undertook some inoculation experi- 
ments, and will give the details of two, which establish the 
life-history of the fungus. (Expt. 10.)— I inoculated a potted 
plant, which I had in confinement since the preceding autumn, 
several times with sporidia during April. On the 23rd I 
noticed two distinct points of attack on one leaf, though as 
yet there was no spore-eruption. This, however, occurred on 
the 29th, when I saw two or three black pustules on each 
spot of attack. On the 9th May I noticed that another leaf 
was wholly attacked, including the whole of the petiole. 
(Expt. 11.) — On this date I removed some spores and placed 
them in water under a glass shade. On the following day 
some had germinated and had produced sporidia (Fig. 3) ; 
but on the 13th (24x4 hours) germination was free and 
complete. 
