On Aggregations of Proteid in the cells of 
Euphorbia splendens. 
BY 
R. E. FRY, B.A. 
With Plate XXIV, 
HILE examining sections of the stem of Euphorbia 
splendens (using spirit-material) I observed in many 
of the cells, particularly in the parenchyma immediately 
surrounding the vascular bundles, certain spherical bodies, 
which refracted light strongly and in some cases had double 
contours, though they were not doubly refracting. 
They stained deeply with the following reagents : — - 
haematoxylin, Hanstein’s violet, eosin, Hoffmann’s blue. 
With iodine they were stained the yellowish brown colour 
characteristic of proteids. 
They were exceedingly insoluble, not being affected by 
saturated NaCl-solution, 5 per cent, potash, hydrochloric or 
acetic acids, or ether. These reactions suggested the presence 
of a coagulated proteid. This view was confirmed by the use 
of Millon’s reagent, and the xanthoproteic test, which gave 
the characteristic red and deep yellow colours. 
I also used a test described by Loew 1 . It consists in 
precipitating the proteid by the action of a solution of 
potassium ferrocyanide and acetic acid, washing out these 
by treatment with 60 per cent, alcohol for half an hour and 
1 Bot. Zeit. No. 18, May, 1884. 
[Annals of Botany, Vol. V. No. XX. November, 1891.] 
Gg 
