4 Kingo Miyabe.—Ou the Life-history of 
cells and twelve in the flasks were made. Except four failures 
with the former, the rest of the cultures were successful. Since 
that time, two more cell- and eight more flask-cultures have 
been prepared. But they were all poorer in growth, and pro- 
duced few or no perithecia on the mycelium. By way of 
comparison, several open cultures on slides and watch-glasses 
were also prepared. 
A large number of young onion-plants were started both 
from seeds and bulbs. The spores were sown on different 
parts of the leaves ; and the pots were kept moist under bell- 
jars, with the exception of a few which were left uncovered. 
The greater part of the young seedlings were badly injured by 
nematoid worms ; but those which survived did not show any 
sign of the attack. Out of the twelve bulbs, the culture on 
only two was successful. The spores, however, grew in both 
cases only on the sheath of the leaves, and not on the active 
green portions. One of them produced the Mcicrosporium- 
spores in small quantity, while the other formed in addition a 
large number of perithecia. These perithecia were arrested in 
growth when some of them were large enough to form para- 
physes in their interior. 
The spores sown in pure water germinated within eight 
hours. The germ-tubes were as a general rule produced 
from the cells situated on the convex portions of the spores. 
The number of the tubes from each spore varied a great deal 
according to its size. In a fully-grown spore, three to six 
tubes were most commonly seen. These tubes or hyphae 
grew rapidly in length, but produced lateral branches rather 
sparingly. The hyphae were colourless and septate, and were 
filled with refractive contents. The branches were slender and 
anastomosed readily with each other or with the main hyphae, 
when they happened to meet. As the hyphae grew in length, 
many of the cells lost the larger part of their contents, which 
were carried from one portion to another, until they settled in 
a certain part of the hyphae. Towards the end of the main 
hypha a very slender transparent branch was usually observed. 
The extremity of this branch regularly formed a closely coiled 
