6 Kingo Miyabe . — On the Life-history of 
glycogen, as ascertained by the iodine test. Their wall soon 
assumed a light yellowish brown colour. 
On these main hyphae, and very rarely on their larger 
branches, some of the earliest perithecia were formed during 
the second and third days of the culture. The first sign of 
their formation was the division of a certain portion of the 
hypha into a row of short cells. One or usually two or more 
of these cells began to swell considerably, and each of them 
sent out one or more hyphal branches, before any division 
took place in them (Fig. 7). The branches were at first very 
slender and hyaline; and they grew very rapidly, anasto- 
mosing with remarkable readiness with each other, and with 
any other hypha which happened to lie in their course. 
.While the branches were thus growing, the initial cell or 
cells continued to swell and divide, first into two by a trans- 
verse or oblique septum, and then into four or more, and so 
on. There seemed indeed to be no regular directions in the 
cell-divisions. The resulting tissue was parenchymatous in 
structure, and the cells composing it were so closely united as 
to leave scarcely any intercellular space between them. The 
basal portions of the hyphal branches mentioned above began 
at the same time to swell and divide (Fig. 8). The groups of 
cells thus formed, coalescing with the central tissues, gave to 
perithecia at this stage most irregular forms with several pro- 
jections (Fig. 9). But as they went on growing and dividing, 
they sent out from the newly formed outside cells an addi- 
tional number of similar branches. Their basal parts again 
by growth and division contributed new groups of cells to the 
growing central mass ; and thus the young perithecia gradually 
assumed a definite globular shape. At the beginning of the 
culture, all the changes and growth took place within 
twenty-four hours in a vigorously growing plant ; while in 
those perithecia formed later on, when the nourishment had 
become somewhat scarce, it took about three or more days 
for a similar amount of growth. 
On the fourth or fifth day after the sowing, the outside 
layer of cells of a young perithecium began to assume a dark 
