of the Genus Doassansia , Cornu. 7 
or seven sporidia, the odd sporidium is found unconjugated, but 
as having put forth the conjugating tube (Figs. 20 and 21). 
The results of conjugation are best seen in the pairs of spo- 
ridia which have fallen from the basidial cell and are floating 
free (Figs. 22 to 31). As far as I have been able to determine, 
a germ-tube is uniformly produced, and it may start from the 
tip of one sporidium (Figs. 23 and 24), from the tips of both, 
or from the base (Figs. 25 to 27)- Various combinations of 
these various methods are common (Figs. 28 to 31). As the 
germ-tube grows, the protoplasm is withdrawn from the spo- 
ridia, and septa are formed as occurred in the promycelium. 
Occasionally the promycelium does not produce a crown of 
sporidia but developes at once into a germ-tube (Fig. 32). 
Cornu did not note the conjugation of the sporidia in D. alis- 
matis ; and Fisch, finding that the sporidia of D. sagittariae 
did not conjugate, says that the germination of the spores in 
the genus Doassansia is like that of the spores of Title tia and 
Entyloma , but lacks the conjugation (Ber. Deutsch. Bot. Gesell., 
Bd. II, p. 415). He also seems to regard the lack of conjuga- 
tion in D. sagittariae as a case of apogamy ( 1 . c., pp. 409 
and 410). 
The first germinations, as mentioned above, were obtained 
from fresh material at Sharon Springs, N. Y., and took place 
in the months of July and August, 1889. The later germina- 
tions from dried material were obtained in October and No- 
vember, 1889, and March, 1890. The dried material was 
soaked over night in water, and the following morning some 
sori were crushed on a slide, a little water added, and the 
slide then placed in a moist chamber. In all the sowings thus 
made, mature sporidia were produced after twenty-four hours. 
Nearly all of the variations figured were obtained in each 
sowing. In no case did I observe the production of secondary 
sporidia, but the sporidia conjugated in pairs and produced 
germ-tubes. The odd sporidium was not seen to germinate 
in any case. 
D. alismatis differs from D. hottoniae both in habit and in 
the structure of the sorus, as noted under that species. It re- 
