Champici par villa, Harv. from the Carpospore. 343 
season reach a large size, the tufts being four to five inches in 
diameter. On these plants the older portions of the frond 
bear empty cystocarps, and the younger portions cystocarps 
which are just mature or in process of development. The ripe 
spores may be easily obtained in large quantities by leaving 
the fruiting plants over night in a dish of sea-water. In the 
morning the bottom of the dish will be sprinkled with the 
reddish spores, which have been thrown out during the night. 
This ejection of the spores occurs only at night, as was proved 
by experiment. Many of the spores, when they escape from 
the cystocarps, settle down on the mother-plant and develop 
there into young plants, not unfrequently reaching the length 
of an inch and a half. Most of the stages in the segmentation 
of the spore (Plate XXI, Figs. 4-10) were obtained from the 
parent plant, and all of the young plants come from the same 
source. There could be no doubt about the identity of these 
small plants with the mother-plant, for one could find them 
in all stages of development, from the newly-shed spore to the 
plants showing all the characteristics of the adult frond, with 
diaphragms and hyphae. 
Several attempts were made to grow the spores, but they 
never developed beyond a 4-celled stage. The method of 
sowing was to place the spores on a glass slide in the bottom 
of a dish of sea-water, and then to keep a small stream of sea- 
water constantly running through the dish. The spores are 
heavy, and readily remain on the slide. Those which ger- 
minated attached themselves to the slide, so that they could 
be killed, hardened, stained, and mounted in balsam, by 
simply dipping the slide into the various fluids. There was 
silt suspended in the salt water, which settled over the spores, 
and probably prevented their farther development. 
The spores, which measure -05—08 mm. in diameter, 
are spherical, and filled with dark red chromatophores 
(Fig. 1). 
The nucleus lies in the centre, making that portion of the 
spore appear lighter and brighter in colour. It was noticed 
that the smaller spores germinated more readily and appeared 
