20 Lister . — Notes on the P lasmodium of 
moisten ed, after three or four weeks, they took a longer time 
to recover the movement. 
The dry sclerotium of Badhamia utricularis is dark brick- 
red in colour, of brittle, horny texture (Fig. 7), and con- 
solidates in irregular effused masses, which are usually made 
up of cord-like convolutions and knobs : the cells of which it is 
composed vary in size from 10 to 20 /x in diameter. 
When a thin section of dry sclerotium is placed in water and 
examined under the microscope, the cells are seen to swell 
from absorption of moisture, and in a short time a slow change 
of position takes place in the contained granules, among 
which may be observed from 5 to 20 nuclei, according to the 
size of the cell (Fig. 8). These are not easy to detect, but 
if the swollen cells are carefully separated and crushed on a 
cover-slip, then dried and stained with magenta, the nuclei, 
with their nucleoli, are brought out with beautiful distinctness, 
especially when mounted in Canada balsam (Fig. 10). 
The sclerotium is often formed with free rods connecting one 
part with another (Fig. 7,0) ; a section of one of these, when 
softened in water, shows very well how the refuse matter is 
discharged by encysting plasmodium ; the outer wall of the 
rod is composed of mucus charged with spores of Fungi and 
cells of Algse, together with other rubbish, while the enclosed 
cells contain only pure plasmodium. 
The sharpest definition of nuclei and nucleoli which I have 
succeeded in obtaining has been when the cover-slips on which 
the plasmodium of Badhamia had been thinly smeared, were 
instantly dropped into absolute alcohol ; the preparations were 
then stained with magenta and mounted in balsam. Stainings 
of hyaloplasm taken from turgid plasmodium show very few 
nuclei, which appear to be confined to the interior substance, 
for the scattered individuals that are met with were probably 
introduced through imperfect manipulation. 
If the streaming plasmodium is examined without having 
resort to staining, and a morsel is placed under a cover-slip 
with slight pressure, the nuclei cannot at first be recognised ; 
