Bctdhamia utricular is and Brefeldia maxima. 2 1 
but as disintegration takes place they gradually become 
visible (Fig. 11, a). 
They are rendered more conspicuous if a bit of plasmodium 
is torn with needles in a drop of water, care being taken that 
the cover-slip does not press too closely ; viewed under a 
magnifying power of about 1200, the globular vesicles of finely 
granular plasma which ooze out from the mass are seen to 
contain nuclei, which are colourless and faint, but at the same 
time perfectly distinct in outline. They measure, as a rule, 
a little over 3 ju, in diameter, and show a clearly defined 
nucleolus. Occasionally a nucleus may be seen to be shot into 
an expanding vesicle in which none had previously appeared, 
as if it had formed an obstruction in the narrow passage through 
which the plasma issued ; some globular vesicles that have 
become detached contain nuclei in great abundance, while 
others again have few or none. 
When an object such as is here described has remained for 
half an hour or so under the cover- slip, the yellow granules 
will be found to have mostly disappeared, the minute granules 
will have more or less dispersed or congregated together, 
while the colourless plasma is seen crowded with nuclei 
throughout the preparation. 
The most successful arrangement for minute observation of 
the streaming plasmodium is obtained when we happen to 
have it climbing freely over the sides of a glass shade. If this 
has been going on for a day or two, we frequently see little 
buddings out from the larger veins of delicate fans of network 
measuring perhaps an eighth of an inch across, or less. A 
drop of water is placed over one of these, and it is gently 
detached, with precaution that no branches are injured except 
the main stem, which connected it with the vein. If the little 
fan be placed in water on a glass slide, and the cover-slip 
supported at one side by a piece of blotting-paper to prevent 
its pressing the plasmodium, it will continue its streaming 
movement without interruption, and will remain in healthy 
condition for some days. If the fan lies in a small bubble of 
air surrounded by water, it will confine its movements very 
