348 Marshall Ward . — On a lily -disease. 
organs of attachment are developed in contact with the glass, 
and conidia are formed on the surface of the sheet, but not in 
large numbers or in big heads, probably owing to the re- 
stricted access of free air. 
With such cultures as these the following series of experi- 
ments were instituted. 
Series I. 
On July 17, three flasks were prepared and sterilised, 
corked with cotton wool, etc. as follows, and conidia of the 
Botrytis sown in them. 
1 = Pasteur’s solution. 
2 = Pasteur’s solution, and a little peptone. 
3 = Fresh urine. 
On August 1, dense mycelial crusts had formed on 
Nos. 1, 2, corrugated and growing out in all directions. 
On No. 3, a mere film had commenced to form, and had 
then perished. 
I then (August 1) filtered 1 the yellow liquid of No. 1 into 
two test-tubes, which had been properly sterilised : these 
tubes may be called A and B respectively. 
I then boiled the liquor in A for two minutes : that in B 
was left untouched. 
Damp chambers, properly sterilised, were then prepared as 
for cultures in hanging drops, and used as follows : — 
a = Two were arranged with the hanging drop of the 
boiled liquor (test tube A) and a thin section of lily- 
bulb-scale placed in the drops. 
(3 = Two others were arranged exactly as above, but the 
drops consisted of the unboiled liquor (test-tube B). 
y — Two were arranged as in a (i. e. the drops consisted 
of boiled liquor), and two spores of the Botrytis placed 
in the drop as well as the lily-bulb-section. 
a = Two others were arranged exactly as in y, but with 
drops of the unboiled liquor. 
1 Not without considerable difficulty. 
