349 
Marshall Ward . — On a lily -disease. 
On August r, at 6 p.m., these damp-chambers were placed 
in a larger moist chamber in the laboratory, the temperature 
being i2°-i5° C. 
On Aug. 2 , at io a.m., I examined the above with the 
microscope. 
The sections in a and y were practically unaltered, but 
those in (3 and b presented a curious appearance, due to the 
peculiar brilliance of their cell-walls : the cellulose was some- 
what swollen, and refracted the light so that the edges of the 
walls appeared coloured. It was evident that something in 
the liquid had caused a change in the cell-walls of such a kind 
that they became diffluent and swollen : moreover this some- 
thing was destroyed by boiling. 
I also devised the following parallel arrangements, as a sort 
of check on the foregoing. 
On Aug. l, eight culture-chambers were prepared as before, 
and the hanging drops constituted as follows : — 
€ = Two of distilled water only. 
C = Two of Pasteur’s solution. 
?? — Two of distilled water, with one or two Botrytis- 
spores in. 
6 = Two of Pasteur’s solution with one or two Botrytis- 
spores. 
These eight cultures were placed in a similar damp- 
chamber to the preceding eight, and at the same time ; and 
they also were examined when the preceding ones were. 
So far as the cell-walls were concerned, i. e. neglecting 
plasmolysis and other easily explained alterations, the 
sections in these cultures showed no change. The spores 
germinated normally. 
I was by no means satisfied with these experiments ; for 
although there was a distinct swelling of the cell-walls in the 
cases marked [3 and 5, it was not quite so conspicuous as in 
my rough experiment described on p. 346 . However, repeti- 
tion of the observations again led to the conclusion that the 
cell-walls did undergo the changes described. 
