35 ° Marshall Ward . — On a lily -disease. 
On Aug. 2, I took the remainder of the Pasteur's liquid 
from flask No. I, and employed it in three parts. 
Part I was gently decanted into a clean test-tube : the 
nearly clear liquor contained a few spores and bits of 
mycelium. 
Part II was filtered with difficulty into a second test-tube, 
yielding a clear yellowish liquor as in previous cases. 
Part III was not only filtered, but also boiled for five 
minutes. 
The tubes were stopped with cotton-wool, and marked 
X, Y, and Z respectively, and in each tube I placed one or 
two short bits, with smooth clean-cut ends, of the bud, leaf, 
peduncle, and bulb of the white lily. 
The tubes were then left until io a.m. on Aug. 4 — i.e. 
about 40 hours — in an ordinary temperature. The changes 
which had then ensued in the test-tubes X and V were 
sufficiently obvious to be seen with the unaided eye, especially 
with the short cylindrical bits of peduncle. 
Whereas those in the tube Z (boiled liquor) still preserved 
their sharp smooth-cut ends and edges, those in X and Y 
(and especially in A) had their ends swollen and gelatinised, 
and projecting both longitudinally and laterally over the cut 
epidermis, so that the pieces looked like dumb-bells, the 
handle being formed of the intact epidermis and cuticle 
compressing the tissues beneath, and the heads of the swollen 
cells radiating at the ends. 
Sections and microscopical examination showed that the 
tissues of the pieces in the test-tube Z were practically un- 
altered ; in X and F, however, the ‘heads’ of the dumb-bell-like 
pieces were composed of the separated swollen cells of the 
cortex and pith. Something in the liquor had in fact caused 
the dissolution of the middle lamella and the gelification of 
the cellulose. That this something is a ferment is not only 
highly probable from the preceding, but becomes almost a 
certainty from the ease with which it is destroyed on boiling 
the liquor. There were no bacteria to be observed in the 
fluid. 
