Marshall Ward. — On a lily -disease. 351 
Having repeated these experiments with like success, it 
seemed probable that better results, i. e. more intense action, 
might be got by employing an aqueous extract of the 
fungus : with this object the following series of experiments 
were made. 
Series II. 
On August 4, I removed with a newly drawn glass hook 
the mycelium from a flask-culture of three weeks’ standing : 
the mycelium was about 4 inches in diameter, and about 
3 mm. thick, grey in colour, and of a tough almost carti- 
laginous texture. This mycelium was then crushed in a 
mortar in its own juice, i. e. with so much of the liquor as it 
had carried away from the flask, and a little distilled water. 
Owing to its slippery, semi-cartilaginous character, it was not 
easily pounded : after the operation the unpleasant-looking 
mess evolved a distinct sickly smell, not evident before 
crushing. 
The whole mess was then poured into a clean flask, and 
the neck stopped with cotton-wool, and allowed to brew for 
an hour. 
The liquor was then poured off and divided into two equal 
parts, as follows: — 
Half was filtered 1 into a small test-tube, labelled, and left 
intact. 
The other half was filtered into a second tube, and boiled 
for five minutes. 
The results were similar to those obtained previously ; slices 
of lily-tissues placed in the unboiled liquor had their cell- 
walls swollen, whereas those in the boiled liquor underwent 
no such change. Again, however, I felt that the amount of 
swelling of the cell-walls was too slight to be absolutely con- 
vincing, so the following method was tried. 
1 The process of filtering was difficult : for the first minute or so the liquor 
came through fairly rapidly, but it afterwards filtered through very slowly indeed. 
Thinking this might be due to some action on the filter-paper, I examined the 
latter subsequently; but I was unable to satisfy myself that the fibres were 
altered. 
