Marshall Ward . — On a lily -disease. 
353 
Series IV. 
A six-weeks’ culture of the Botrytis, on Pasteur’s solution 
with a little peptone, was removed from its flask, and the thick 
fungus-crust pounded in a mortar with a little distilled water : 
the mess was then allowed to digest for a few hours at 28 0 - 
30° C. It was then filtered, the filtered liquor falling into a 
tall tube, and four or five times its bulk of alcohol was added. 
Contact with the alcohol at once caused a milkiness, which 
rapidly increased on agitation ; and in less than a minute a 
series of flocculent white masses formed throughout the liquid, 
some of which slowly sank to the bottom, others floated 
quietly to the top. The tube was left to stand all night, at 
the ordinary temperature, and in the morning the white bulky 
flocks had increased a little, and numerous small tufts of a like 
nature were sticking to the sides of the tube. 
I removed some of the floating flocculent masses, and 
examined them with the microscope : they consisted partly of 
amorphous substance reminding one of fibrine, and partly of 
crystalline substance of at least two kinds I thought at first 
that the crystals might possibly be those of tyrosin chiefly, 
with some leucine, but that idea was not supported by their 
examination by a chemical friend. Be this as it may (and it 
is of secondary importance because the crystals etc. only act 
as a vehicle), the partly amorphous, partly crystalline flocks, 
after being dried at the ordinary temperature over sulphuric 
acid in the partially exhausted receiver of an air-pump, formed 
a greyish mass, which swelled and partly dissolved in distilled 
water. 
I placed some of it in a small watch-glass, with a little dis- 
tilled water, and allowed it to digest two hours ; I then added 
a few sections of the pedicel of Lilium auratum (being unable 
to obtain Lilium candidum), and allowed them to soak for 
eight hours at 30° C. The results were as before — the middle 
lamellae of all the parenchyma cells were destroyed, and the 
cells isolated as if they had been boiled, while the cellulose- 
