377 
Marshall Ward. — On a lily-disease. 
conidia in water ; the larger one measured mm. long by mm. broad. At a is 
a conidium germinating after lying three hours in water at the ordinary tempera- 
ture ; a' the same four and a-half hours later ; a" the same fifteen hours later still. 
Zeiss D. 
Fig. 12. A similar culture in water. The conidium /3 was placed in water at 
11.30 a.m. on July 7th ; at 2.30 p.m. it had germinated as shown. / 3 ' the same at 
7 p.m. ; / 3 " the same at 10 a.m. next day (July 8) ; the same at 7 p.m. on 
July 8th. It will be noticed that as the process of germination advanced, the 
protoplasm in the spore became very frothy, and finally passed entirely into the 
hyphae (e. g. / 3 " and 16'"). Still later 08 "'), the protoplasm in the hyphae also 
became vacuolated. On the 9th of July all growth had ceased, the culture 
remaining as shown in / 3 "', except that the vacuolation had increased : and by 
July nth (i. e. after nearly five days in water) the septate hyphae contained little 
ut water and a few granules. The tip of the long hypha had swollen a little. 
All Zeiss D. 
Fig. 13. A conidium germinating in water, in which a slice of a lily-bud had 
been placed ; the influence of the food-material was at once evident from the outset 
in the more numerous and stronger branches, etc. The spore was sown on August 
1 st, at 4 p.m., and the drawing made August 2nd, 11 a.m. Zeiss D. 
Fig. 14. Germination in Pasteur’s solution. The spore a was sown on July 7, at 
7 p.m., and when drawn (to a.m., July 8th), had been germinating for 15 hours. 
Zeiss D. 
PLATE XXI. 
Fig. 15. The same conidium nine hours subsequently, the culture going on in a 
hanging drop of Pasteur’s solution. Zeiss D. 
Fig. 16. The same culture on July 9th at 8.30 a.m., i. e. 1 3^ hours subsequently. 
The drawing was made as exact as possible, but under a lower power (Zeiss B), in 
order to get the whole in. It will be noticed that at three places, marked x , 
cross-connections have been established between the hyphae. 
Fig. 17. The same culture 48 hours later still, i. e. after four days from germina- 
tion. A few of the ends of main hyphae have been omitted, but otherwise the 
courses of the branches are shown as correctly as possible : numerous cross- 
connections are observable, and certain small groups of tendril-like thin hyphae 
should be noted. The spore is still to be seen to the left below. Zeiss B. 
Fig. 18. Two of the tufted groups of thin tendril-like hyphae of the above, more 
highly magnified. Zeiss D. 
Fig. 19. A portion of the above mycelium, showing the cross-connections 
established by the fusion or conjugation of neighbouring hyphae. Note the 
remarkable case near x , where two short branches from below have curved over, 
and fused with one from above ; while two other short branches, curving over still 
more towards the same point, have failed to establish the connection. Zeiss D. 
Fig, 20. Portions of the mycelium of Fig. 11, showing the characteristic outlines 
of the tendril-like endings of the branches : the two slightly swollen ends, x x , 
are beginning to stick to the glass. Only the outlines are drawn, but the whole of 
the branches were full of bright protoplasm, and rapidly growing. Zeiss D. 
Fig. 21. One of the curious swollen endings of hyphae in various stages of 
growth. The mycelium was three days old, and growing in Pasteur’s solution. 
a was the condition at 2.30 p.m. ; £ = 3.30 p.m. ; £ = 6.30 p.m., and d-= 10.30 p.m. 
C C 
