378 Marshall Ward, — On a lily-disease. 
It is these swollen, club-shaped endings which form the ‘ organs of attachment.’ 
Zeiss D. 
Fig. 22. One of the organs of attachment seen from above, as it adheres to the 
cover-slip from which the culture-drop was hanging. Its cell-walls deliquesce, and 
glue themselves flat on to the glass, as shown in profile in Fig. 23. Such an organ 
often puts forth a branch at x (Fig. 23), and goes on growing thence. Zeiss J. 
Fig. 24. Three of the organs of attachment viewed from above, through the 
glass as before, showing how they may branch, and go on growing. Zeiss E. 
Fig. 25. Young organs of attachment branching to form the tufts shown in 
Fig. 15 e. Zeiss D. 
Fig. 26. Medium-sized (by no means the largest) tassel-like organs of attach- 
ment produced by the repeated branching of smaller ones. Zeiss D. 
PLATE XXII. 
Fig. 27. The end of a hypha from the same mycelium as Fig. 15. a represents 
the condition of affairs at 7 p.m. on July 18 ; ^ = 6.30 a.m. on the 19th ; c — 10 a.m. 
(19th) ; d= 11.40 a.m. ; e was drawn at 12.30 ; f at 12.45 5 S at 12 -55 5 and ^ at 
1. 10 p.m. The chief point of interest is the gradual outgrowth of the branch, 
x (in d ), and its fusion with another one. The branch x appeared as a slight pro- 
tuberance about 11.30 a.m., and in 10 minutes presented the condition figured in d ; 
at 12 o’clock the small branch opposite to it appeared, and was as shown in e at 
12.30. It is noteworthy that the direction of the upper branch x changed, and it 
curved so as to point the apex towards the new protuberance. As the two apices 
approached, the growth became more rapid, and at 12.55 (<£") the tips were nearly 
in contact. Absolute contact took place at 1.3, as nearly as I could determine; at 
1. 10 the protoplasm of the two branches was continuous, as in h. The system was 
watched up to the evening, but no further changes of importance were noticed. 
Zeiss D. 
Fig. 28. Another case where the ends of two hyphae, at first growing at an 
angle, gradually turn so as to grow in the same straight line and fuse, a was drawn 
at 10 a.m., b at 10.45, and c at 12.30. As before, the protoplasm eventually 
became continuous by the fusion of the apices. Zeiss D. 
Fig. 29. Another case of the same kind, but less accurately timed. A was the 
condition of affairs at 4 p.m., and B was drawn at 8 p.m. ; but it should be noted 
that the junction was already complete, at the point marked x . Zeiss D. 
Fig. 30. At 12.45 a junction was already established where the two hyphae cross 
at x (in a), and just below two branches are growing out towards one another ; to 
the right is a long, thin, straight hypha shooting across from another branch ; at 
2.25 the end of this straight hypha was in contact with one of the first-named 
branches (0 in / 3 ), and soon after it effected a junction. The effect of this was 
apparently to stop the further development of the two short branches near o ; and 
they did not join. The mycelium from which this preparation was taken was 
cultivated in thick raisin-extract. Zeiss D. 
Fig. 31. Taken from same mycelium as last, a was drawn at 4 p.m., and b 
at 4.10 p.m. The tip of the upper branch slid slowly along the lower branch, but 
no junction was established between them. In some cases junction is established 
eventually at the point of crossing after 24-48 hours. Such cases seem to prove 
that the hyphae must be in some special condition before they can establish junc- 
tions. Zeiss D. 
