380 Marshall Ward. — On a lily -disease. 
solution. At 11.30 a.m. it presented the appearance shown in a , having six septa 
and a long growing segment above the sixth septum: at 3.20 p.m. this apical 
segment alone had grown to double its former length and had formed two new 
septa, as shown in b. Zeiss D, oc. 4. 
Fig. 40. Further preparations giving some information as to the growth of the 
filaments. In a, the hypha lay just above a very thin section of epidermis, at 
2.55 p.m. At 3.5 p.m. the tip of the hypha had moved forward as shown in b: 
at 3-3° p.m. the tip had progressed further still, having passed over the distance 
shown in c, and a small lateral branch had begun to form below. A comparison 
of the figures suggests that the apex described a slightly sinuous course, or very 
open spiral : it will be understood that the section of epidermis (part of a much 
larger piece) lay in a deeper plane. Zeiss D. 
Fig. 41. The hypha a, crossing the one b, was in the position figured — relative 
to the diamond-shaped intercellular space towards which its tip is pointing — at 
3.2 p.m., and it traversed the distances marked by x x in ten minutes, reaching 
the first x at 3.7, and the second x at 3.12. Zeiss D. 
Fig. 42. Three characteristic groups of terminal branches of a mycelium grown 
for a week in Pasteur’s solution : the whole mycelium was produced from one spore 
At the points marked x , the ends of the hypha are seen to give off each a small 
cloudy drop. This, when first excreted, is a transparent, apparently mucilaginous 
drop, with very minute brilliant granules in it : as it ages, however, in a few hours 
it becomes more granular and has a yellowish hue. It gives proteid reactions, 
and the evidence goes to show that it contains a ferment which liquefies cellulose. 
Zeiss D. 
Fig. 43. Similar excretions of drops from a culture in thin extract of raisins. 
Note the vacuolation of the hyphae. Zeiss D. 
Fig. 44. Similar excretions of drops from hyphae of a large culture in Pasteur’s 
solution (seven days after sowing). The preparation was taken from the edges of the 
large circular mycelium, which was a millimetre thick and nearly a decimetre in 
diameter : the excretion is much more copious in such pure cultures in flasks, 
with plenty of air and food-materials. Zeiss D, oc. 4. 
Fig. 45. One of the free hyphae of a strong culture shown in process of ex- 
cretion of the drop. At first the protoplasm is bright and almost homogeneous 
( 1 ) ; when the drop commences to exude, the protoplasm has been getting more 
and more vacuolated and granidar (2) ; and this increases ( 3 ) ; until at length there 
may be very little protoplasmic content left (4) ; and the end may even collapse ; 
but it frequently happens that the hypha goes on growing in the stage 3, either 
putting out a bud-like outgrowth which becomes a branch, or itself going on 
growing. Zeiss J, oc. 3 and 4. 
Fig. 46. Preparation showing the infection of the epidermis of a lily-bud. 
The conidium was placed on the bud in a drop of distilled water two days pre- 
viously : it had germinated, and the tip of the longer tube attached itself closely to 
the cuticle and then discoloured and pierced it, the contents of the spore passing 
into the tube. The epidermal cells are only in outline. Zeiss D, oc. 2. 
Fig. 47. Similar preparation, with two infecting conidia : in the upper one the 
whole of the protoplasm of the spore has already passed into the infecting tube, in 
the lower one some is still retained. Forty-eight hours’ germination. As shown by 
