On the development of the Aleurone-grains 
in the Lupin. 
BY 
A. B. RENDLE, B.A., 
Si, John’s College , Cambridge . 
With Plate X. B. 
formation of aleurone-grains, the characteristic 
1 proteid reserve-material found in seeds, was studied 
by Pfeffer 1 sixteen years ago. According to his results, 
the mineral contents, crystals of calcium oxalate, or the 
‘ globoids ’ of double phosphate of lime and magnesia, first 
make their appearance in the cell-sap, and then, singly or 
in groups, act as centres of attraction for the proteid matter, 
which, as the seed in ripening loses water, is precipitated 
from the turbid cell-sap. Where proteid crystalloids occur, 
they too appear in the cell-sap simultaneously with the 
inorganic solids. 
In describing their development in L,upin (referring more 
especially to L. polyphyllus), Pfeffer says, ‘The protoplasmic 
strands having been converted into ground-substance, the 
resulting arrangement might at first sight easily suggest the 
idea that the protoplasm becomes a parenchymatous network 
whose meshes form moulds for the immigrating metaplasmic 
substance. But the history of development is opposed to 
such a conclusion.’ 
It would appear, however, at any rate in Lupinus digitatas 
which has been investigated in the present instance, that this 
1 Pringsheim’s Jahrb. fur Wissenschaft. Bot. Bd, 8. 1872, 
[Annals of Botany, VoL II, No, VI, August 1888, ] 
