A leur one-grains in the Lupin . 163 
continuity with the rest of the protoplasm and enclosing 
the cavities from which the soluble matter has been abstracted 
(Fig. 2 ). It is therefore evident that the above-mentioned 
bodies consist of some substance, presumably proteid, soluble 
in dilute potash, which has* been secreted by and in the 
protoplasm. 
If sections be similarly treated with 10 per cent, or saturated 
solutions of common salt or potassium phosphate, the bodies 
merely swell up somewhat but are not dissolved, and, if 
washed in water, even after lying for twenty hours in the 
salt solutions, appear quite unaltered. 1 per cent, and 10 
per cent, solutions of hydrochloric acid, even after twenty 
hours’ action, only cause slight swelling. The bodies therefore 
differ in solubility from the grains of the ripe seed, which are 
completely and at once soluble in such solutions. 
After solution a perfectly clear space is seen to remain, 
and there is no sign whatever of crystalline or globoid 
contents. 
These bodies, which, as the sequel shows, are the primitive 
aleurone-grains, increase in size and number and soon fill up 
the vacuole, so that the cell contains within the parietal layer 
of protoplasm a number of roundish grains quite separated 
from each other by a protoplasmic reticulum, made up of the 
bridles and the membranes originally separating the secretion 
from the vacuole. By watching a section in which this stage 
has not quite been reached, while dilute potash is run under 
the slip, the limiting protoplasmic membranes of adjacent or 
opposite masses of the secretion are seen to swell out and 
meet to form what has now every appearance of a proto- 
plasmic strand, indicating how the same would take place in 
the ordinary process of growth. Near the centre, or sometimes 
at the side, is seen the nucleus, which is becoming more or 
less compressed by the growing grains ; these relations are 
clearly brought out by iodine, and the protoplasmic network 
demonstrated by running in dilute potash which at once 
dissolves the grains, leaving quite empty cavities. 
By the time the vacuole has been nearly filled up, a dif- 
