1 66 Rendle . — On the development of 
aleurone-grains described here corresponds most closely with 
the manner of secretion of mucilage as lately described 1 by 
Gardiner and Ito in the glandular hairs of Blechnum and 
Osmunda ; in both cases the secretion is strictly intrapro- 
toplasmic, both the aleurone-grains and mucilage-drops more- 
over remaining, after secretion, quite separate in a reticulum 
of protoplasm. In both cases too there is some chemical 
change in the originally secreted substance, before the final 
product is formed. 
The seeds used in these investigations were preserved in 
absolute alcohol ; o, per cent, chromic acid material shows 
the early stages very well, but as the grains begin to increase 
in size, the cells are seen to be full of empty rings, an ap- 
pearance which is maintained up to the time when the seed 
is fully ripe ; the grains are moreover rendered quite insoluble, 
even in the ripe seed, in salt solutions and 5 per cent, potash. 
By placing sections of the ripe seeds, preserved in alcohol, in 
1 per cent, of chromic acid solution, the homogeneous grains 
are converted into rings, which now resist for several minutes 
the action of 5 per cent, potash and remain undissolved, even 
after twenty hours, in saturated salt solution. 
The development of aleurone-grains in general is obviously 
not completely indicated above, as no account is taken of the 
time and manner of appearance of the globoid and crystalloid, 
which may both be present, as e. g. in Ricinus communis , 
though Lupinus digitatus has neither. I hope to work out 
these points also, in the summer, when material can be 
procured. 
To judge from the title 2 , which alone I have seen, and 
that only a few days since, my results agree with those 
arrived at in a paper by Wakker. 
1 Annals of Botany, I. 1. 1887. 
2 * Aleuronkorrels zijn vacuolen,’ in Maandblad voor Natuurwetenschappen, 
Nos. 5 and 6, 1887. 
