A leur one-grains in the Lupin . 
167 
EXPLANATION OF FIGURES IN PLATE X. b. 
Illustrating Mr. Rendle’s paper on the development of Aleurone -grains in the 
Lupin. 
Fig. 1. First stage in formation of aleurone-grains in Lupinus digitatus. Drawn 
from a preparation stained with Hofmann’s blue. Portion m, unshaded, not in 
focus. The bodies mentioned in the text are line-shaded. Zeiss’ D objective and 
ocular 4. n, the nucleus. 
Fig. 2. Same stage as the last, showing the little pockets in the protoplasm from 
which the secretion has been dissolved out by dilute KOH. Now in iodine. The 
swollen starch-grains shaded dark, a and b same magnification as in Fig. 1. c under 
F objective, n, the nucleus. 
Fig. 3. A little older than the above, grains filling up the vacuole, a and b both 
from preparations stained with Hofmann’s blue ; b, after action of dilute KOH 
showing the protoplasmic network. D objective, ocular 4. Colourless starch- 
grains seen in the protoplasm lining the wall. 
Fig. 4. The growing grains largely fill the cell. In it are seen the grains 
vacuolated as described in the text, and colourless starch-grains in protoplasm, 
after staining with Hofmann’s blue, b, after solution of grains with dilute KOH, 
and staining of protoplasmic network with iodine. Swollen starch-grains lining 
the wall, n , the nucleus. D objective, ocular 4. c a little older than it. 
Fig. 5. Shows progress of solution of two vacuolated grains, in dilute KOH on 
the left, in 10 per cent, of salt solution on the right. D objective, ocular 4. 
Fig. 6. From a nearly ripe seed, a, a cell before, b, one after action of dilute 
KOH. a shows the deeply and homogeneously stained grains, b the protoplasmic 
matrix and nucleus, n. The colourless drops are oil. D objective, ocular 4. 
Fig. 7. From a quite ripe seed stained with Hanstein’s solution. Nucleus and 
protoplasmic matrix have stained a deep violet, the wall a lighter colour, the 
grains almost perfectly colourless, n, nucleus. D objective, ocular 2. 
