2 o 6 Hartoz . — Recent Researches 
is formed, filled with this substance. The convex terminal 
wall or ‘cap ’ is duller and less sharp-contoured than the rest 
of the sporangial wall ; its boundary not being distinct 
from the protoplasm on its inner side. The hyaloplasma 
plug soon becomes granular, except a thin layer lining the cap 
of the process. [The protoplasm of the apex of all growing 
hyphae is hyaline ; in all cases this * hyaloplasma 5 shows 
granules on treatment with iodine.] 
The segregation of the zoospores proceeds thus. In normal 
sporangia appear numerous splits in the protoplasmic invest- 
ment, stopping just short of the cell-wall and opening into 
the vacuole ; these appear and disappear, and finally become 
constant forming a honeycomb network. At first numerous 
plasmatic bridges connect the origins so mapped out ; but 
most of these soon disappear ; it is to the optical expression 
of these bridges that we must refer Biisgen’s ‘ Kornerplatten 
this is especially clear in Achlya. Some protoplasm may 
remain long distinct from the ‘ origins,’ apart from the con- 
tinuous wall. 
In full sporangia the appearance of a zigzag slit indicates 
the segregation of the origins in the smaller sporangia ; in 
the larger the segregation is produced by the appearance of 
linear lacunae (Spalten) which form a connected system. 
In poor sporangia the segregation rather takes place by 
the aggregation of protoplasm in heaps, at the expense and 
by the thinning of the intervening part of the parietal layer. 
Here also plasmatic bridges may occur, and some fragments 
of protoplasm are left out of the schema. (In Aphanomyces 
the spore-origins appear as bulgings of the parietal layer of 
protoplasm, which meet and form transverse disks, joined 
by the intervening thin annular portions of the parietal 
layer.) Rothert describes these elevations as shifting, rising 
and flattening out for some time before becoming stable ; 
but I feel sure that this is a misinterpretation of the gradual 
‘ rotation 5 of the protoplasmic lining of the sporange as a 
whole, carrying the origins with it, which may also be well 
observed in thin ‘ full ’ sporangia of my Saprolegnia ( Lep - 
