Vines— Proteolytic Enzymes in Plants (//). 607 
each solution were placed in each of 8 bottles, and to each (except 
one, the control) one of the following antiseptics was added : NaF, 
1 % ; Salicylic acid, 1 %; thymol, -5 % ; chloroform, -5% ; toluol, -5 % ; 
formalin, *8 %. 
After 7 hours’ digestion at 40° C., the tryptophane-reactions were : — 
Distinct ; HCN bottle. 
Faint; thymol, toluol, control, chloroform. 
None; Salicylic acid, NaF, formalin. 
1 7 hours later, the reactions were : 
Strong ; HCN. 
Distinct ; thymol, toluol, control. 
Faint ; Salicylic acid, chloroform. 
None ; NaF, formalin. 
A similar series of experiments, in which fibrin (2 grms.) 
replaced the Witte-peptone, and in which a stronger solution 
of papain (4 grms. extracted with 200 cc. dist. water) was 
used, gave confirmatory results. 
Experiment with Fibrin. 
The same antiseptics in the same strength as before. After 7 hours’ 
digestion, the results were : — 
HCN ; fibrin completely disintegrated ; distinct tryptophane 
reaction. 
Salicylic acid ; fibrin unaffected ; no tryptophane. 
Thymol ; fibrin slightly attacked ; no tryptophane. 
NaF ; fibrin partly disintegrated; no tryptophane. 
Chloroform ; fibrin unaffected ; no tryptophane. 
Toluol; fibrin gelatinous ; no tryptophane. 
Formalin ; fibrin unaffected ; no tryptophane. 
Control ; fibrin largely disintegrated ; distinct tryptophane. 
19 hours later, the results were essentially similar: the fibrin was 
rather more attacked in one or two cases, but it had not been com- 
pletely disintegrated in any but the HCN and the control bottles, and 
these were still the only bottles the contents of which gave any 
tryptophane-reaction, strong in the HCN bottle, distinct in the control. 
The contents of all the bottles gave good biuret-reaction. 
In these experiments the influence of HCN in promoting 
proteolysis by papain is very evident. In order to determine 
