250 Vines. — Proteolytic Enzymes in Plants. 
The only other Grass that I have investigated so far is Phalaris 
canariensis , and it gave even more definite results than Holcus. 
Apium graveolens. 
I experimented with the Celery with the special object of ascer- 
taining whether or not the green leaf-blades and the etiolated petioles 
would give concordant results : I found this to be the case. 
Experiment 1. — 10 grms. of bruised green leaf-blades were placed 
in each of three bottles (40 cc.), together with 0-3 grm. of Witte- 
peptone : No. 1 was filled up with chloroform-water (0-5 %) ; No. 2 
was filled with distilled water that had been boiled with the leaf- 
material before the Witte-peptone was added ; No. 3 was filled with 
o* 1 % HCN. 
After 22 hours’ digestion, No. 1 gave a marked tryptophane-reaction, 
and No. 3 a strong one; No. 2 gave no reaction. 
Experiment 2. — 10 grms. of bruised etiolated petioles were placed 
in each of three bottles, the other contents of which were precisely 
the same as in the preceding experiment. 
After 5 hours’ digestion, No. 1 gave a distinct, and No. 3 a faint, 
tryptophane-reaction; after 23 hours’ digestion the reaction in No. 1 
was strong, marked in No. 3, and faint in No. 2. 
Scolopendrium vulgare. 
An experiment with the leaves of the Hart’s Tongue Fern, as 
representing the Pteridophyta, is of peculiar interest. 
5 grms. of bruised leaf were placed in each of three bottles (40 cc.) ; 
to each 0*3 grm. of Witte-peptone was added, and the bottles were 
filled up with distilled water ; the leaf in No. 2 had been boiled before 
being placed in it; to No. 3, 1-5 cc. of 4 % HCN were added. 
After 24 hours’ digestion, slight indications of the tryptophane- 
reaction were given by Nos. 1 and 3; after 42 hours’ digestion, 
No. 1 gave a strong reaction, and had an offensive smell; No. 2 
gave no reaction ; No. 3 a marked reaction. 
Bulbs. 
Experiments were made with the Tulip, the Hyacinth, and 
the Onion, the material used being pieces of the bulbs. 
Tulipa , sp. 
5 grms. of bulb in each of three bottles (45 cc.) : all three were 
filled up with chloro form- water ; the bulb-material in No. 2 had 
