256 Vines. — Proteolytic Enzymes in Plants . 
Similar results were obtained with the Mushroom, 5 grms. 
of the solid substance being used. 
Less marked evidence of digestion of milk was obtained 
with Orange-peel, and with the bulbs of the Tulip and the 
Hyacinth, as also with the Banana. No digestion was ob- 
served with the Turnip, the Vegetable Marrow, the Pear, the 
Apple, with the leaves of the Cabbage, the Lettuce, and the 
Spinach, or with the ‘milk’ of the Coco-nut, in 19 hours. 
These experiments generally included a control-bottle con- 
taining the mixture of chloroform-water and milk, without 
any vegetable substance at all : in no case did the contents of 
the control give any trace of tryptophane-reaction. 
Tryptophane in Plants. 
It will have been observed that, in the foregoing account of 
the experiments, mention is incidentally made of the presence 
of tryptophane in the expressed juices or in the watery 
extracts of plants : for instance, in the case of the Banana, 
the Melon, the yellow (but not the green) Cucumber, the 
Vegetable Marrow, the Tomato, among fruits ; of the bulb of 
the Onion (strong), and of the root of the Turnip. I have not 
found it in the juice of the Orange, the Apple, or the Grape, 
nor in extracts of the tubers of the Potato and the Jerusalem 
Artichoke, of Green Peas, of Wheat-Germ, or of any of the 
shoots or leaves mentioned. 
I have made a few further observations upon the occurrence 
of this substance, and have found it in the ‘ milk ’ of the 
Coco-nut ; in extracts of seedlings of the Bean ( Vicia Faba) 
2-3 inches in height, of the Scarlet Runner (. Phaseolus 
maltiflorus ) 6-8 inches in height, and of the Pea (. Pisum 
sativum ) a foot high, excluding the cotyledons in all cases. 
I have not found it in seedlings of the Maize, apart from the 
seed : nor in the shoots of the Asparagus, nor in etiolated 
shoots produced by germinated tubers of the Potato and of 
the Jerusalem Artichoke, though it was present in the potato- 
shoots after they had turned green in consequence of exposure 
to light. 
