382 Howard. — On some Diseases of the 
cultures of this fungus, adopting the precautions described 
above in the experiments with Melanconium . In two of the 
canes inoculation was made with spores, in the other two 
with growing mycelium from pure cultures. Four similar 
canes were used as controls. On January 32, one of each 
of these canes was examined. At the lower internodes of 
each of the inoculated canes the mycelium had spread about 
12 inches above and below the puncture, and macro-conidia 
were developed in the hollow centre of the cane. The 
affected tissues were slightly reddish, and the odour of ethyl 
acetate was very marked. At the upper part of the cane 
the fungus had, in each case, completely spread through the 
infected internodes, the tissues of which were black on account 
of the development of large numbers of macro-conidia in 
the cells. The control canes showed no infection. On 
January 27, the rest of the canes were examined. Both the 
inoculated canes gave practically the same results as those 
examined five days earlier. 
2. On December 26, spores from a pure culture were placed 
on the uninjured leaf-bases at upper and lower nodes of two 
canes. On January 2, one of these canes was examined, when 
it was found that infection had taken place at the two nodes. 
At the lower node, the mycelium of the fungus had invaded 
about 3 feet of the cane. Macro-conidia were developed 
in the centre, and the odour of ethyl acetate was evident. 
At the upper node about 6 inches of the stem were infected, 
and the, tissues were black through an excessive development 
of macro-conidia in the cells. The other cane was examined 
on January 27, when it was found that infection had only 
taken place at the upper node, when about 8 inches of the 
cane were invaded as in the previous case. 
3. At the same time spores and food-material were placed 
on the uninjured internodes of four sound canes and covered 
up with waxed tape. A month afterwards no infection could 
be detected. 
The result of these experiments leaves no doubt that 
Thielaviopsis is capable of pronounced parasitism on the 
