386 Howard. — On some Diseases of the 
In tubes containing 10 c.c. of the cane-extract, a similar 
development occurred, except that stromata appeared on the 
surface in from four to five days. 
In none of the cultures were any further spore develop- 
ments noted, and no trace of the formation of any of the 
phases of Trichosphaeria Sac chari, Massee, was detected. 
It now remained to perform inoculation experiments with 
pure cultures of this fungus on healthy sugar-canes. These 
were as follows : — 
1. On December 4, six healthy canes in the same stool 
were inoculated at wounds made in internodes about the 
middle of the stem and also at upper leaf-bases, with spores 
from a pure culture of the fungus. The precautions, described 
above, to prevent the entry of other spores were taken, and 
six other canes were used as controls. On December 10, 
one of the inoculated canes showed that infection was taking 
place at the wound, but no result was observed at the leaf- 
base. On December 16, a second cane was examined, when 
distinct infection was observed in the tissues of the internode 
where the wound was made and also at the upper leaf-base. 
On December 26, two more canes were examined. No infec- 
tion was detected at the leaf-bases, but at the wounds very 
definite indications of the ‘ rind ’ disease were noted. The 
leaves were beginning to dry in the characteristic manner, 
and on splitting open the canes infection was apparent in 
four of the internodes, where the red blotches, with white 
centres, were evident. The invading mycelium was charac- 
terized by its branching and oil-drops, and agreed exactly 
with that seen in canes attacked by the c rind ’ disease. The 
remaining two canes were also drying at the top and were 
obviously infected at the wounds. They were used for the 
experiments with Melanconium described below. In this 
experiment one of the controls became infected with the 
fungus ; the other five gave negative results. 
2. On December 10, six canes were inoculated in a similar 
manner to those in the above experiment, and six others were 
used as controls. On December 28, one of the inoculated 
