Sugar-Cane in the West Indies. 387 
canes showed infection at the wound, but not at the leaf-base. 
On January 22, two of the inoculated canes showed that at 
the wounds the fungus had invaded two of the internodes 
and had produced the characteristic red blotches with white 
centres. In one case infection had also taken place at a 
leaf-base. The other three canes, in which infection at the 
wounds was very evident, were used for the experiments 
with Melanconium described below. The control canes gave 
negative results. 
3. On December 19, four canes were doubly inoculated 
at wounds made in an upper and a lower internode, with 
mycelium from a pure culture of the Colie totrichum. As 
before, controls were employed and precautions taken to 
introduce only one fungus. The object of this experiment 
was to determine the comparative effect of the fungus on 
those portions of the cane which are very rich and very poor 
in sugar. On January 22, a cane was examined, when it 
was found that the fungus had invaded 16 inches of the 
upper part, which showed the characteristic markings, but 
had not spread beyond the internode at the lower wound. 
The remainder of the canes were examined five days later. 
In all cases infection had taken place to about the same 
extent, the length of cane affected varying from 18 to 24 
inches. The characteristic red blotches with white centres 
were abundant. 
4. On December 31, four canes were inoculated with spores 
from a pure culture of the Colletotrichum as follows. In two 
cases the canes were doubly inoculated at upper and lower 
leaf-bases, and in the other cases at wounds in upper and 
lower internodes. Two control canes were also used. On 
January 22, the canes which had been inoculated at leaf-bases 
showed that infection had taken place at both the upper 
nodes and at one of the lower nodes, At the upper part of 
both canes the stromata of the fungus were abundant on the 
affected rind at the nodes above and below the point of 
inoculation. In each case about 9 inches of the cane were 
affected and the red blotches were abundant. A similar 
