388 Howard . — On some Diseases of the 
result was observed in the case of the cane where the fungus 
had also infected at a lower node but no stromata were 
evident on the rind. On January 23, the canes inoculated 
at wounds showed that in all cases infection had taken place, 
and stromata had formed on the outside at the upper 
affected regions. From 12 to 18 inches of the cane were 
invaded at each wound. The controls gave negative results. 
The above inoculation experiments were carried out with 
canes during the ripening period and after active growth in 
size had ceased. The results obtained, while indicating that 
the fungus is a wound parasite, nevertheless do not conclu- 
sively show that it is capable of overcoming tissues still 
capable of growth and development. Accordingly, further 
experiments were made on plant-canes 1 about six months 
old which were in a vigorous state of growth. In all cases 
inoculation was performed in developing internodes which 
were then not more than 1 inch in length. The experi- 
ments were as follows : — 
5. On June 20, three young canes were inoculated by 
placing seven days old, actively growing, mycelium, from 
a pure culture in the sugar-cane extract medium, into wounds 
made in the centre of a lower internode then about three- 
quarters of an inch in length. Care was taken to introduce 
only one fungus and to shut off the apertures from the air by 
means of sterilized waxed tape. Three similar canes were 
used as controls. Two months afterwards the canes were 
examined. In the first case, the infected internode had grown 
to inches in length, and on splitting open the cane this 
and the internode below were found to be generally reddish 
in colour with the elliptical white areas, characteristic of 
the ‘ rind ’ disease, well represented. About 4 inches of the 
cane were invaded by mycelium, which agreed with that of 
C. falcatum. A closely similar result was obtained in the 
other two inoculated canes, but the controls showed no 
infection. 
1 The first crop of canes raised from cuttings are known in the West Indies as 
‘ plant-canes.’ 
