400 Howard . — On some Diseases of the 
enclosed a medulla of thin-walled, parallel, septate, colourless 
mycelium. The dark strands shaded off into loose, white, 
feathery growths. These latter are very common in cultures 
and also on cane-cuttings infected with this fungus. They 
differ markedly from those associated with the sclerotia of 
Went’s ‘red-rot’ disease. 
In no case were toadstools obtained in the above cultures. 
They were, however, produced in the infection experiments 
described below. 
With one exception three series of inoculation experiments 
were made in which the infecting material was obtained from 
different sources in each case. Thus, in addition to using 
the cultures obtained from a single toadstool spore and those 
from the white mycelium on the leaf-sheaths, pieces of 
the leaf-sheath, obtained from diseased canes, were also 
employed. 
Experiment i. In the first instance, three young rattoon 
shoots, about a foot high, were selected for the experiment. 
After washing with water, portions of six days’ old mycelium 
(developed from a spore) growing in the cane-extract medium 
given above, were placed in the axil of one of the lower leaves 
of two of the shoots and the whole was covered with a clean 
lamp chimney, cotton-wool being packed around the shoot 
at the upper end. The third shoot was used as a control. 
In seven days, the leaf-sheath, on which the mycelium was 
placed, began to turn yellowish-red, and in fourteen days the 
whole of this and the next sheath above were covered with 
a white film of matted mycelium. Reddish areas, where the 
fungus had invaded and destroyed the tissues, were abundant, 
and the leaves attached to these sheaths were rapidly drying up. 
Sections through the red portions of the leaf-sheaths showed 
that the cells were penetrated by a mycelium, characterized 
by clamp-connexions and dark-brown, thick-walled, chlamydo- 
spore-like bodies. The control shoot gave no results. A 
closely similar set of events followed when culture-mycelium, 
obtained from the leaf-sheaths of diseased canes, and portions 
of these leaf-sheaths themselves were employed. 
