401 
Stigar-Cane in the West Indies . 
Experiment 2. Three two-eyed cuttings were selected from 
the upper part of healthy White Transparent canes, carefully 
washed and placed on moist coral sand, previously sterilized, 
in flower-pots standing in dishes containing water. These 
served to keep away ants. The pots were then covered with 
glass bell-jars and placed in a plant shed. Seven days after- 
wards, when the cuttings had sprouted, two of them were 
infected at the cut ends with mycelium (developed from 
a toadstool spore) similar to that used in experiment 1 above, 
and the buds were also covered therewith. The third cutting 
was not infected and thus served as a control. The cuttings 
were now covered with moist sand. In one case, in seventeen 
days, and in the other in twenty-nine days after infection, small 
white circular bodies, about the size of a pin’s head, were 
noted at the surface of the sand on the lower leaf-sheaths 
of a shoot. In forty-eight hours these developed into toad- 
stools identical with those obtained on cane-shoots attacked 
by root disease. On examining these shoots it was found 
that the lower leaf-sheaths were dead and cemented closely 
by white mycelium to those underneath. The outermost of 
those still living were covered with mycelium and showed 
numerous reddish areas, where the cells were found to be 
invaded by hyphae. The control cutting showed no infection. 
A similar result was obtained with culture-mycelium from the 
leaf-sheaths of diseased canes, and in one case toadstools 
appeared three weeks after infection. With portions of the 
leaf-sheaths from diseased canes a limited amount of infection 
was obtained, but no toadstools were produced and the shoots 
seemed to suffer little from the fungus. During this experi- 
ment the cuttings were watered, after the appearance of green 
leaves, with Sachs’ solution. 
Experiment 3. Experiment 2 was next repeated, except that 
sterile soil was used in the flower-pots, and the cuttings were 
infected when planted, and watered throughout with boiled 
tap-water. The developing shoots were found to be attacked 
by the fungus, but in one case only (when pure culture- 
mycelium had been employed) were toadstools developed 
