52 
PACIFIC SCIENCE, Vol. XV, January 1961 
Fig. 3. Chromatogram of free amino acids of Haliotis cracherodii taken from White’s Point, a polluted 
hours for each portion. Extracts were then con- 
centrated and stored under refrigeration as a 4.4 
ml. solution of 10 per cent isopropanol. 
Chromatograms were run on 1814- by 2214- 
inch Whatman no. 1 filter papers. A mixture of 
butanol, acetic acid, and water (4:1:5) was 
used as the first phase; water-saturated phenol 
was used as the second. Development was car- 
ried out by dipping in a 0.2 per cent solution 
of ninhydrin in acetone. 
RESULTS 
The abalone, Haliotis cracherodii, from both 
polluted and nonpolluted areas contained the 
amino acids alanine, arginine, aspartic acid, 
cystine, glutamic acid, glycine, histidine, leucine- 
isoleucine, tyrosine, and valine. Specimens taken 
from the nonpolluted areas (Santa Catalina 
Island, San Clemente Island, and Anacapa Is- 
land) contained asparagine in addition to the 
above amino acids. The specimens taken from 
White’s Point showed no asparagine, but did 
show a definite spot identified as aspartic acid. 
This acid was either absent or only very faintly 
discernible in the specimens from the non- 
polluted areas. Phenylalanine and three uniden- 
tified spots were present in the specimens ob- 
tained from the White’s Point area (Figs. 2, 
3; Table 1). 
All specimens of the crab, Pachygrapsus 
crassipes, contained the free amino acids alanine, 
arginine, aspartic acid, cystine, glutamic acid, 
glycine, histidine, leucine isoleucine, lysine, me- 
thionine, proline, serine, threonine, tyrosine, and 
